Dina Julia, Creveuil Christian, Gouarin Stephanie, Viron Florent, Hebert Amelie, Freymuth Francois, Vabret Astrid
Department of Virology, CHU de Caen, F-14000 Caen, France.
Normandy University, Faculty of Medicine, UNICAEN, EA4655, F-14000 Caen, France.
Viruses. 2016 Aug 20;8(8):234. doi: 10.3390/v8080234.
The objective of this study is primarily to compare the performance of the VIDAS(®) Measles immunoglobulin (Ig)G assay to that of two other serological assays using an immunoassay technique, Enzygnost(®) Anti-measles Virus/IgG (Siemens) and Measles IgG CAPTURE EIA(®) (Microimmune). The sensitivity and the agreement of the VIDAS(®) Measles IgG assay compared to the Enzygnost(®) Anti-measles Virus/IgG assay and the Measles IgG CAPTURE EIA(®) assay are 100%, 97.2% and 99.0%, 98.4%, respectively. The very low number of negative sera for IgG antibodies does not allow calculation of specificity. As a secondary objective, we have evaluated the ability of the VIDAS(®) Measles IgG assay to measure anti-measles virus IgG antibody avidity with the help of the VIDAS(®) CMV IgG Avidity reagent, using 76 sera from subjects with measles and 238 other sera. Different groups of populations were analyzed. In the primary infection measles group, the mean IgG avidity index was 0.16 (range of 0.07 to 0.93) compared to 0.79 (range of 0.25 to 1) in the serum group positive for IgG antibodies and negative for IgM. These data allow to define a weak anti-measles virus IgG antibody avidity as an avidity index (AI) < 0.3 and a strong avidity as an AI > 0.6. The VIDAS(®) Measles IgG assay has a performance equivalent to that of other available products. Its use, individual and quick, is well adapted to testing for anti-measles immunity in exposed subjects.
本研究的主要目的是使用免疫测定技术，将VIDAS（®）麻疹免疫球蛋白（Ig）G检测法与另外两种血清学检测法，即Enzygnost（®）抗麻疹病毒/IgG（西门子公司）和麻疹IgG捕获酶免疫测定法（Microimmune）进行性能比较。与Enzygnost（®）抗麻疹病毒/IgG检测法相比，VIDAS（®）麻疹IgG检测法的灵敏度和一致性分别为100%、97.2%，与麻疹IgG捕获酶免疫测定法（Measles IgG CAPTURE EIA）（®）相比分别为99.0%、98.4%。IgG抗体阴性血清数量极少，无法计算特异性。作为次要目的，我们使用76份麻疹患者血清和238份其他血清，借助VIDAS（®）巨细胞病毒IgG亲和力试剂，评估了VIDAS（®）麻疹IgG检测法测量抗麻疹病毒IgG抗体亲和力的能力。分析了不同人群组。在原发性感染麻疹组中，IgG亲和力指数平均为0.16（范围为0.07至0.93），而在IgG抗体阳性且IgM阴性的血清组中为0.79（范围为0.25至1）。这些数据有助于将抗麻疹病毒IgG抗体低亲和力定义为亲和力指数（AI）<0.3，高亲和力定义为AI>0.6。VIDAS（®）麻疹IgG检测法的性能与其他现有产品相当。其使用方便快捷，非常适合对暴露人群进行抗麻疹免疫力检测。
