几内亚蒲桃大果变种树皮提取物对大鼠匀浆中三乙酸铁诱导应激的体外器官保护作用
In vitro organo-protective effect of bark extracts from Syzygium guineense var macrocarpum against ferric-nitrilotriacetate-induced stress in wistar rats homogenates.
作者信息
Tankeu Francine Nzufo, Pieme Constant Anatole, Biapa Nya Cabral Prosper, Njimou Romain Jacques, Moukette Bruno Moukette, Chianese Angelo, Ngogang Jeanne Yonkeu
机构信息
Department of Biochemistry and Physiological Sciences; Faculty of Medicine and Biomedical Sciences, Laboratory of Biochemistry, University of Yaoundé I, PO Box 1364, Yaounde, Cameroon.
Laboratory of Medicinal plant Biochemistry, Food Science and Nutrition, Department of Biochemistry, Faculty of Science, University of Dschang, PO Box 67, Dschang, Cameroon.
出版信息
BMC Complement Altern Med. 2016 Aug 26;16(1):315. doi: 10.1186/s12906-016-1263-1.
BACKGROUND
Overconsumption of oxygen in mammalian cells often lead to the production of reactive oxygen species (ROS) resulting from different mechanisms. Escape of scavenging enzymes/components or nutritional failure are the most important origins. Plant-derived molecules may protect biological molecules either by quenching free radicals, delaying or preventing the ROS formation or by restoring antioxidant enzymes activities. The present study assessed the antioxidant, phenolic profile and protective effect of barks extracts of Syzyguim guineense var macrocarpum against ferric nitriloacetate-induced stress in the liver, heart kidney and brain tissues of wistar rat homogenates.
METHODS
Three extracts (aqueous, ethanol and aqueous-ethanol) from the barks of S. guineense var macrocarpum were used in this study. The spectrophotometric standardized methods were used to determine the free radical scavenging and antioxidant potential of the extracts. The protective properties of these plant extracts were also investigated as well as the quantification of secondary metabolites content (total phenolic, flavonoids and flavonols content). The HPLC method helped for characterizing phenolic compounds present in these extracts.
RESULTS AND DISCUSSION
All the extracts exhibited a free radical scavenging potential in a concentration dependent manner which varied from 15.18 ± 0.80 to 97.15 ± 0.71 % depending to the type of extract and the method used. The ethanol extract had the higher phenolic content (432.85 mg QE/g extract), including total flavonoids (961.66 mg QE/g extract) and flavonols content (25.12 mg QE/g extract) and higher total antioxidant capacity. Among the phenolic compounds present in the extracts, the HLPC profile revealed the presence of syringic acid and apigenin in all the extracts. The extracts demonstrated their protective effect mostly in liver and brain homogenates by delaying or preventing lipid peroxidation, restoring enzymatic activities and enhancing glutathione levels.
CONCLUSION
The overall results demonstrated that the extracts exhibited significant antioxidant and protective effects in liver and brain liver homogenates.
背景
哺乳动物细胞中氧气的过度消耗通常会通过不同机制导致活性氧（ROS）的产生。清除酶/成分的逃逸或营养缺乏是最重要的来源。植物衍生分子可以通过淬灭自由基、延迟或防止ROS形成或恢复抗氧化酶活性来保护生物分子。本研究评估了几内亚蒲桃大果变种树皮提取物对硝酸铁诱导的Wistar大鼠肝脏、心脏、肾脏和脑组织匀浆应激的抗氧化、酚类成分及保护作用。
方法
本研究使用了几内亚蒲桃大果变种树皮的三种提取物（水提取物、乙醇提取物和水 - 乙醇提取物）。采用分光光度标准化方法测定提取物的自由基清除和抗氧化潜力。还研究了这些植物提取物的保护特性以及次生代谢物含量（总酚、黄酮类和黄酮醇含量）的定量。HPLC方法有助于表征这些提取物中存在的酚类化合物。
结果与讨论
所有提取物均呈现出浓度依赖性的自由基清除潜力，根据提取物类型和使用方法的不同，清除率在15.18±0.80%至97.15±0.71%之间变化。乙醇提取物的酚类含量较高（432.85 mg QE/g提取物），包括总黄酮（961.66 mg QE/g提取物）和黄酮醇含量（25.12 mg QE/g提取物），且总抗氧化能力较高。提取物中存在的酚类化合物中，HPLC图谱显示所有提取物中均存在丁香酸和芹菜素。提取物主要通过延迟或防止脂质过氧化、恢复酶活性和提高谷胱甘肽水平，在肝脏和脑组织匀浆中表现出保护作用。
结论
总体结果表明，提取物在肝脏和脑组织匀浆中表现出显著的抗氧化和保护作用。
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