Department of Surgery, Clinical Medicine, The Affiliated Clinical College Second People's Hospital of Shenzhen, Anhui Medical University, Hefei, Anhui 230032, P.R. China.
Toxicology Research Laboratory, The Shenzhen Center for Disease Control and Prevention, Shenzhen, Guangdong 518055, P.R. China.
Mol Med Rep. 2016 Oct;14(4):2975-82. doi: 10.3892/mmr.2016.5645. Epub 2016 Aug 19.
The expression and functions of microRNA (miR)-411 have been investigated in several types of cancer. However, until now, miR-411 in human breast cancer has not been examined. The present study investigated the expression, biological functions and molecular mechanisms of miR‑411 in human breast cancer, discussing whether it offers potential as a therapeutic biomarker for breast cancer in the future. The expression levels of miR‑411 in human breast cancer tissues and cells were measured using reverse transcription‑quantitative polymerase chain reaction analysis. Following transfection with miR‑411 mimics, an MTT assay, cell migration and invasion assay, western blot analysis and luciferase assay were performed in human breast cancer cell lines. According to the results, it was found that miR‑411 was significantly downregulated in breast cancer, and associated with lymph node metastasis and histological grade. Additionally, it was observed that miR‑411 suppressed cell growth, migration and invasion in the breast cancer cells. The present study also provided the first evidence, to the best of our knowledge, that miR‑411 was likely to directly target specificity protein 1 in breast cancer. These findings indicated that miR‑411 may be used a therapeutic biomarker for the treatment of breast cancer in the future.
miR-411 的表达和功能已在几种类型的癌症中进行了研究。然而，到目前为止，人类乳腺癌中的 miR-411 尚未被检测到。本研究探讨了 miR-411 在人类乳腺癌中的表达、生物学功能和分子机制，探讨其是否有潜力成为未来乳腺癌的治疗性生物标志物。采用逆转录-定量聚合酶链反应分析检测人乳腺癌组织和细胞中 miR-411 的表达水平。转染 miR-411 模拟物后，在人乳腺癌细胞系中进行 MTT 检测、细胞迁移和侵袭检测、Western blot 分析和荧光素酶检测。结果表明，miR-411 在乳腺癌中显著下调，与淋巴结转移和组织学分级有关。此外，观察到 miR-411 抑制乳腺癌细胞的生长、迁移和侵袭。本研究还首次提供了证据，据我们所知，miR-411 可能直接靶向乳腺癌中的特异性蛋白 1。这些发现表明 miR-411 可能在未来被用作治疗乳腺癌的治疗性生物标志物。
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