Fieldhouse Sarah, Oravcova Eliska, Walton-Williams Laura
Staffordshire University, Department of Forensic and Crime Science, The Science Centre, Leek Road, Stoke on Trent ST4 2DF, England, United Kingdom.
Forensic Sci Int. 2016 Oct;267:78-88. doi: 10.1016/j.forsciint.2016.08.003. Epub 2016 Aug 10.
The recovery of DNA and fingermark evidence from the same site can be problematic on account of potential contamination from fingermark visualisation techniques, and/or the destructive capability of the DNA recovery method. Forensic investigators are therefore often required to choose which evidence type to recover, or to recover both evidence types from different sites. Research typically documents the effects of fingermark visualisation techniques on the subsequent recovery of DNA, whereas this research has investigated the effects of DNA recovery on the quality of subsequently recovered latent fingermarks. Eccrine rich, sebaceous rich, and 'normal' latent fingermarks were deposited onto five substrates: glass; aluminium; textured plastic; varnished wood; photocopier paper and aged from 4h to 4 weeks. Approximately half of the control fingermarks were developed without DNA recovery on all substrates. The remaining samples were subjected to one of five DNA recovery methods prior to fingermark development. Pre and post DNA recovered fingermarks were graded for quality, and AFIS correlations scores were obtained and analysed for statistically significant differences using Wilcoxon Signed Rank tests and Friedman tests. All of the DNA recovery methods reduced the quality of latent fingermarks on glass surfaces. Flocked swabs and gel lifts were the least destructive DNA recovery methods on the remaining surfaces, except for aluminium sheet metal. The quality of latent fingermarks deposited onto glossed wood and textured plastic and paper were less affected by dry swabbing. Wet swabbing and tape lifting were very damaging methods of DNA recovery.
由于指纹可视化技术可能造成污染,和/或DNA提取方法具有破坏性,从同一地点提取DNA和指纹证据可能会出现问题。因此,法医调查人员常常需要选择提取哪种证据类型,或者从不同地点提取这两种证据类型。以往研究通常记录指纹可视化技术对后续DNA提取的影响,而本研究调查了DNA提取对后续提取的潜在指纹质量的影响。将富含外分泌汗腺、富含皮脂腺和“正常”的潜在指纹沉积在五种基质上:玻璃;铝;纹理塑料;涂漆木材;复印纸,并放置4小时至4周使其老化。在所有基质上,大约一半的对照指纹在不进行DNA提取的情况下进行显影。其余样本在指纹显影前采用五种DNA提取方法之一进行处理。对提取DNA前后的指纹进行质量分级,并获得AFIS相关性分数,使用Wilcoxon符号秩检验和Friedman检验分析是否存在统计学显著差异。所有DNA提取方法均降低了玻璃表面潜在指纹的质量。除铝板外,植绒拭子和凝胶提取是在其余表面上破坏性最小的DNA提取方法。沉积在有光泽木材、纹理塑料和纸张上的潜在指纹质量受干拭子的影响较小。湿拭子和胶带提取是极具破坏性的DNA提取方法。