透析废液中的4-吡哆酸：对荧光和光学监测的贡献。

4-Pyridoxic Acid in the Spent Dialysate: Contribution to Fluorescence and Optical Monitoring.

作者信息

Kalle Sigrid, Tanner Risto, Arund Jürgen, Tomson Ruth, Luman Merike, Fridolin Ivo

机构信息

Department of Biomedical Engineering, Technomedicum, Tallinn University of Technology, Tallinn, Estonia.

Centre of Nephrology, North Estonian Medical Centre, Tallinn, Estonia.

出版信息

PLoS One. 2016 Sep 6;11(9):e0162346. doi: 10.1371/journal.pone.0162346. eCollection 2016.

DOI:10.1371/journal.pone.0162346

PMID:27598005

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5012589/

Abstract

AIM

In this work we estimated the contribution of the fluorescence of 4-pyridoxic acid (4-PA) to the total fluorescence of spent dialysate with the aim of evaluating the on-line monitoring of removal of this vitamin B-6 metabolite from the blood of patients with end-stage renal disease (ESRD).

METHODS

Spectrofluorometric analysis of spent dialysate, collected from hemodialysis and hemodiafiltration sessions of 10 patients receiving regularly pyridoxine injections after dialysis treatment, was performed in the range of Ex/Em 220-500 nm. 4-PA in dialysate samples was identified and quantified using HPLC with fluorescent and MS/MS detection.

RESULTS

Averaged HPLC chromatogram of spent dialysate had many peaks in the wavelength region of Ex320/Em430 nm where 4-PA was the highest peak with contribution of 42.2±17.0% at the beginning and 47.7±18.0% in the end of the dialysis. High correlation (R = 0.88-0.95) between 4-PA concentration and fluorescence intensity of spent dialysate was found in the region of Ex310-330/Em415-500 nm, respectively.

CONCLUSION

4-PA elimination from the blood of ESRD patients can be potentially followed using monitoring of the fluorescence of the spent dialysate during dialysis treatments.

摘要

目的

在本研究中，我们评估了4-吡哆酸（4-PA）荧光对透析废液总荧光的贡献，旨在评估对终末期肾病（ESRD）患者血液中这种维生素B-6代谢产物清除情况的在线监测。

方法

对10例在透析治疗后定期接受吡哆醇注射的患者的血液透析和血液透析滤过过程中收集的透析废液进行荧光光谱分析，检测范围为激发波长/发射波长220 - 500 nm。采用带荧光和串联质谱检测的高效液相色谱法对透析液样本中的4-PA进行鉴定和定量。

结果

透析废液的平均高效液相色谱图在激发波长320/发射波长430 nm的波长区域有许多峰，其中4-PA是最高峰，在透析开始时其贡献为42.2±17.0%，透析结束时为47.7±18.0%。在激发波长310 - 330/发射波长415 - 500 nm区域，发现4-PA浓度与透析废液荧光强度之间具有高度相关性（R = 0.88 - 0.95）。