检测大鼠创伤性脑损伤后的行为缺陷

Detecting Behavioral Deficits Post Traumatic Brain Injury in Rats.

作者信息

Awwad Hibah O

机构信息

Department of Pharmaceutical Sciences, University of Oklahoma College of Pharmacy, 1110 N. Stonewall Ave. CPB 315, Oklahoma, OK, 73117, USA.

Oklahoma Center for Neuroscience, College of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.

出版信息

Methods Mol Biol. 2016;1462:573-96. doi: 10.1007/978-1-4939-3816-2_31.

DOI:10.1007/978-1-4939-3816-2_31

PMID:27604739

Abstract

Traumatic brain injury (TBI), ranging from mild to severe, almost always elicits an array of behavioral deficits in injured subjects. Some of these TBI-induced behavioral deficits include cognitive and vestibulomotor deficits as well as anxiety and other consequences. Rodent models of TBI have been (and still are) fundamental in establishing many of the pathophysiological mechanisms of TBI. Animal models are also utilized in screening and testing pharmacological effects of potential therapeutic agents for brain injury treatment. This chapter details validated protocols for each of these behavioral deficits post traumatic brain injury in Sprague-Dawley male rats. The elevated plus maze (EPM) protocol is described for assessing anxiety-like behavior; the Morris water maze protocol for assessing cognitive deficits in learning memory and spatial working memory and the rotarod test for assessing vestibulomotor deficits.

摘要

创伤性脑损伤（TBI），从轻度到重度，几乎总会在受伤个体中引发一系列行为缺陷。其中一些由TBI引起的行为缺陷包括认知和前庭运动缺陷以及焦虑和其他后果。TBI的啮齿动物模型一直（并且仍然）是确立TBI许多病理生理机制的基础。动物模型还用于筛选和测试治疗脑损伤的潜在治疗药物的药理作用。本章详细介绍了针对Sprague-Dawley雄性大鼠创伤性脑损伤后每种行为缺陷的验证方案。描述了高架十字迷宫（EPM）方案用于评估焦虑样行为；莫里斯水迷宫方案用于评估学习记忆和空间工作记忆中的认知缺陷，以及转棒试验用于评估前庭运动缺陷。