用于泛素蛋白质组鉴定与分析的管柱-质谱联用技术
TUBEs-Mass Spectrometry for Identification and Analysis of the Ubiquitin-Proteome.
作者信息
Azkargorta Mikel, Escobes Iraide, Elortza Felix, Matthiesen Rune, Rodríguez Manuel S
机构信息
Proteomics Platform CICbioGUNE, CIBERehd, ProteoRed-ISCIII, Parque Tecnologico de Bizkaia, Derio, Spain.
Computational and Experimental Biology Group, Department of Health Promotion and Chronic Diseases, National Health Institute Dr. Ricardo Jorge, INSA, I.P., Av Padre Cruz, 1649-016, Lisboa, Portugal.
出版信息
Methods Mol Biol. 2016;1449:177-92. doi: 10.1007/978-1-4939-3756-1_9.
Mass spectrometry (MS) has become the method of choice for the large-scale analysis of protein ubiquitylation. There exist a number of proposed methods for mapping ubiquitin sites, each with different pros and cons. We present here a protocol for the MS analysis of the ubiquitin-proteome captured by TUBEs and subsequent data analysis. Using dedicated software and algorithms, specific information on the presence of ubiquitylated peptides can be obtained from the MS search results. In addition, a quantitative and functional analysis of the ubiquitylated proteins and their interacting partners helps to unravel the biological and molecular processes they are involved in.
质谱分析法（MS）已成为蛋白质泛素化大规模分析的首选方法。目前存在多种用于定位泛素位点的方法，每种方法都有不同的优缺点。我们在此展示一种用于通过TUBEs捕获泛素蛋白质组并进行后续数据分析的质谱分析方案。使用专用软件和算法，可以从质谱搜索结果中获得有关泛素化肽段存在的特定信息。此外，对泛素化蛋白质及其相互作用伙伴进行定量和功能分析，有助于揭示它们所参与的生物学和分子过程。
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