Faraji Shahrivar Farzaneh, Badavi Mohammad, Dianat Mahin, Mard Ali, Ahangarpour Akram, Samarbaf-Zadeh Alireza
Physiology Research Center, Department of Physiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Endocrine Physiology Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Physiology Research Center, Department of Physiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Gene. 2016 Dec 20;595(1):25-30. doi: 10.1016/j.gene.2016.09.025. Epub 2016 Sep 20.
The most important conditions associated with hypothyroidism is the cardiac dysfunction. Apelin is an endogenous ligand, involved in energy storage and metabolism which improves cardiac contractility. This study was done to evaluate the effects of apelin, l-Thyroxin (T4) or a combination of both, on cardiac function and mRNA expression of two contractile proteins, α and β myosin heavy chain (α-MHC and β-MHC), in 6-propyl-2-thiouracil (PTU)-induced hypothyroid rats. Forty male Wistar rats were randomly assigned into five groups: Ctrl (Control), and 4 hypothyroid groups (H, HA, HT, and HAT). The Hypothyroid (H) group received 0.05% PTU in the drinking water for six weeks; the next 3 groups, along with PTU, received apelin (HA, 200μg/kg/day, ip), T4 (HT, 20μg/kg/day, gavage), or a combination of both drugs (HAT) for the last 2weeks (weeks 5 and 6). TSH and T4 were measured using ELISA kit. Isolated hearts of animals were perfused in Langendorff apparatus and left ventricular developed pressure, cardiac contractility, heart rate, rate pressure product and perfusion pressure were assessed using PowerLab ADInstruments. In addition α-MHC and β-MHC mRNA expression were evaluated by RT-PCR method in heart tissue. Apelin alone or accompanied by T4 significantly increased cardiac contractility and performance as compared to hypothyroid group. Apelin also significantly increased the alpha-MHC mRNA expression and in the presence of T4 significantly decreased beta-MHC mRNA expression. It seems that apelin alone may improve cardiac function in hypothyroid rats via genomic pathways.
与甲状腺功能减退相关的最重要的情况是心脏功能障碍。Apelin是一种内源性配体，参与能量储存和代谢，可改善心脏收缩力。本研究旨在评估Apelin、左旋甲状腺素（T4）或两者联合使用对6-丙基-2-硫氧嘧啶（PTU）诱导的甲状腺功能减退大鼠心脏功能以及两种收缩蛋白α和β肌球蛋白重链（α-MHC和β-MHC）mRNA表达的影响。40只雄性Wistar大鼠被随机分为五组：对照组（Ctrl）和4个甲状腺功能减退组（H、HA、HT和HAT）。甲状腺功能减退（H）组在饮用水中给予0.05% PTU，持续六周；接下来的3组在给予PTU的同时，在最后2周（第5周和第6周）分别给予Apelin（HA，200μg/kg/天，腹腔注射）、T4（HT，20μg/kg/天，灌胃）或两种药物联合使用（HAT）。使用ELISA试剂盒测量TSH和T4。将动物的离体心脏在Langendorff装置中进行灌注，并使用PowerLab ADInstruments评估左心室舒张末压、心脏收缩力、心率、速率压力乘积和灌注压力。此外，通过RT-PCR方法评估心脏组织中α-MHC和β-MHC mRNA的表达。与甲状腺功能减退组相比，单独使用Apelin或联合T4可显著增加心脏收缩力和性能。Apelin还显著增加α-MHC mRNA的表达，在T4存在的情况下显著降低β-MHC mRNA的表达。似乎单独使用Apelin可能通过基因组途径改善甲状腺功能减退大鼠的心脏功能。
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