局灶性和全身性癫痫患者血清miR-106b和miR-146a升高。

Elevated serum miR-106b and miR-146a in patients with focal and generalized epilepsy.

作者信息

An Ning, Zhao Wei, Liu Yancui, Yang Xiaofan, Chen Pei

机构信息

Department of neurology (section II), HongQi Hospital, MuDanJiang Medical College, China.

Department of anatomy, MuDanJiang Medical College, China.

出版信息

Epilepsy Res. 2016 Nov;127:311-316. doi: 10.1016/j.eplepsyres.2016.09.019. Epub 2016 Sep 26.

DOI:10.1016/j.eplepsyres.2016.09.019

PMID:27694013

Abstract

BACKGROUND

Epilepsy is a chronic neurological disorder characterized by recurring seizures. Although scalp electroencephalograms (EEG) and neuroimaging have been used in clinical diagnosis of epilepsy, the more economical, rapid and non-invasive biomarker is still desirable, contributing to the accurate clinical diagnosis and facilitating the appropriate treatment.

METHODS

The expression of four epilepsy-associated miRNAs (miR-106b, miR-146a, miR-194-5p and miR-301a) was measured by quantitative RT-PCR in the serum of 90 epilepsy patients and control populations.

RESULTS

It was found that the serum miR-106b, miR-146a and miR-301a were significantly increased but serum miR-194-5p was significantly decreased in epilepsy patients compared with healthy control populations. In addition, serum miR-106b (r=0.6412) and miR-146a (r=0.5896) were correlated with NHS3 score in epilepsy patients. Furthermore, the ROC result of serum miR-106b for prediction of epilepsy was 0.786, higher than those of serum miR-146a (AUC=0.774), miR-194 (AUC=0.686) or miR-310a (AUC=0.696). The combination of serum miR-106b and miR-146a gained a better sensitivity/specificity for prediction of epilepsy (AUC=0.887).

CONCLUSION

Our preliminary findings indicate that upregulated serum miR-106b and miR-146a might be a potential biomarker for epilepsy evaluation.

摘要

背景

癫痫是一种以反复发作性癫痫发作为特征的慢性神经系统疾病。尽管头皮脑电图（EEG）和神经影像学已用于癫痫的临床诊断，但仍需要更经济、快速且无创的生物标志物，以有助于准确的临床诊断并促进适当的治疗。

方法

通过定量逆转录聚合酶链反应（RT-PCR）检测90例癫痫患者和对照人群血清中四种癫痫相关微小RNA（miR-106b、miR-146a、miR-194-5p和miR-301a）的表达。

结果

发现与健康对照人群相比，癫痫患者血清miR-106b、miR-146a和miR-301a显著升高，而血清miR-194-5p显著降低。此外，癫痫患者血清miR-106b（r = 0.6412）和miR-146a（r = 0.5896）与NHS3评分相关。此外，血清miR-106b预测癫痫的ROC结果为0.786，高于血清miR-146a（AUC = 0.774）、miR-194（AUC = 0.686）或miR-310a（AUC = 0.696）。血清miR-106b和miR-146a联合使用对癫痫预测具有更好的敏感性/特异性（AUC = 0.887）。