利用CRISPR/Cas基因组编辑技术在水稻中进行靶向诱变

Use of CRISPR/Cas Genome Editing Technology for Targeted Mutagenesis in Rice.

作者信息

Xu Rongfang, Wei Pengcheng, Yang Jianbo

机构信息

Key Laboratory of Rice Genetics Breeding of Anhui Province, Rice Research Institute, Anhui Academy of Agricultural Sciences, 40# Nongke South Road, Hefei, 230031, China.

出版信息

Methods Mol Biol. 2017;1498:33-40. doi: 10.1007/978-1-4939-6472-7_3.

DOI:10.1007/978-1-4939-6472-7_3

PMID:27709567

Abstract

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated protein (Cas) system is a newly emerging mutagenesis (gene-editing) tool in genetic engineering. Among the agriculturally important crops, several genes have been successfully mutated by the system, and some agronomic important traits have been rapidly generated, which indicates the potential applications in both scientific research and plant breeding. In this chapter, we describe a standard gene-editing procedure to effectively target rice genes and to make specific rice mutants using the CRISPR/Cas9 system mediated by Agrobacterium transformation.

摘要

成簇规律间隔短回文重复序列（CRISPR）/CRISPR相关蛋白（Cas）系统是基因工程中一种新兴的诱变（基因编辑）工具。在重要农作物中，该系统已成功使多个基因发生突变，并快速产生了一些具有重要农艺性状的变异，这表明其在科学研究和植物育种中均具有潜在应用价值。在本章中，我们描述了一种标准的基因编辑程序，该程序可通过农杆菌介导的转化，利用CRISPR/Cas9系统有效靶向水稻基因并创制特定的水稻突变体。