Sarvaiya Hetal A, Lazar Iulia M
Department of Biological Sciences, Virginia Tech, 1981 Kraft Drive, Blacksburg, VA 24061, USA.
Data Brief. 2016 Sep 22;9:579-584. doi: 10.1016/j.dib.2016.09.025. eCollection 2016 Dec.
The proteome data provided in this article were acquired from MCF7 breast cancer cells stimulated with insulin, and were generated by using a 2D-SCX (strong cation exchange)/RPLC (reversed phase liquid chromatography) separation protocol followed by tandem mass spectrometry (MS) detection. To facilitate data re-processing by more advanced search engines and the extraction of additional information from already existing files, both raw and processed data are provided. The sample preparation, data acquisition and processing protocols are described in detail. The raw data relate to work published in "Proteome profile of the MCF7 cancer cell line: a mass spectrometric evaluation" (Sarvaiya et al., 2006) [1] and are made available through the PRIDE (PRoteomics IDEntifications)/ProteomeXchange public repository with identifier PRIDE: PXD004051 ("2016 update of the PRIDE database and tools" (Vizcaino et al., 2016) [2]).
本文提供的蛋白质组数据取自用胰岛素刺激的MCF7乳腺癌细胞,通过二维强阳离子交换/反相液相色谱(2D-SCX/RPLC)分离方案,随后进行串联质谱(MS)检测生成。为便于更先进的搜索引擎进行数据再处理以及从现有文件中提取更多信息,同时提供了原始数据和处理后的数据。详细描述了样品制备、数据采集和处理方案。原始数据与发表于《MCF7癌细胞系的蛋白质组图谱:质谱评估》(Sarvaiya等人,2006年)[1]的研究相关,并通过PRIDE(蛋白质组鉴定)/ProteomeXchange公共存储库提供,标识符为PRIDE:PXD004051(《PRIDE数据库和工具的2016年更新》(Vizcaino等人,2016年)[2])。