水貂中UMPH2、APRT、PEPD、PEPS、PSP和PGP基因的染色体及区域定位：与人类和小鼠的比较
Chromosomal and regional localization of the genes for UMPH2, APRT, PEPD, PEPS, PSP, and PGP in mink: comparison with man and mouse.
作者信息
Pack S D, Zhdanova N S, Sukoyan M A, Serov O L
机构信息
Institute of Cytology and Genetics, Academy of Sciences of the USSR, Siberian Department, Novosibirsk.
出版信息
Cytogenet Cell Genet. 1989;50(2-3):127-31. doi: 10.1159/000132740.
Segregation of mink biochemical markers uridine 5'-monophosphate phosphohydrolase-2 (UMPH2), adenine phosphoribosyltransferase (APRT), phosphoserine phosphatase (PSP), phosphoglycolate phosphatase (PGP), peptidases D (PEPD) and S (PEPS), as well as mink chromosomes, was investigated in a set of mink x mouse hybrid clones. The results obtained allowed us to make the following mink gene assignments: UMPH2, chromosome 8; PEPD and APRT, chromosome 7; PEPS, chromosome 6; and PSP and PGP, chromosome 14. The latter two genes are the first known markers for mink chromosome 14. For regional mapping, UMPH2 was analyzed in mouse cell clones transformed by means of mink metaphase chromosomes (Gradov et al., 1985) and also in mink x mouse hybrid clones carrying fragments of mink chromosome 8 of different sizes. Based on the data obtained, the gene for UMPH2 was assigned to the region 8pter----p26 of mink chromosome 8. The present data is compared with that previously established for man and mouse with reference to the conservation of syntenic gene groups and G-band homoeologies of chromosomes in mammals.
在一组水貂与小鼠的杂交克隆中，研究了水貂生化标记物尿苷5'-单磷酸磷酸水解酶-2（UMPH2）、腺嘌呤磷酸核糖转移酶（APRT）、磷酸丝氨酸磷酸酶（PSP）、磷酸乙醇酸磷酸酶（PGP）、肽酶D（PEPD）和S（PEPS）以及水貂染色体的分离情况。所获得的结果使我们能够进行以下水貂基因定位：UMPH2位于第8号染色体；PEPD和APRT位于第7号染色体；PEPS位于第6号染色体；PSP和PGP位于第14号染色体。后两个基因是水貂第14号染色体上已知的首批标记物。为了进行区域定位，在通过水貂中期染色体转化的小鼠细胞克隆中（格拉多夫等人，1985年）以及在携带不同大小水貂第8号染色体片段的水貂与小鼠杂交克隆中对UMPH2进行了分析。根据所获得的数据，UMPH2基因被定位到水貂第8号染色体的8pter----p26区域。本文的数据与先前针对人类和小鼠所确定的数据进行了比较，涉及哺乳动物中同线基因群的保守性以及染色体的G带同源性。
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