Quagliariello Andrea, Aloisio Irene, Bozzi Cionci Nicole, Luiselli Donata, D'Auria Giuseppe, Martinez-Priego Llúcia, Pérez-Villarroya David, Langerholc Tomaž, Primec Maša, Mičetić-Turk Dušanka, Di Gioia Diana
Laboratory of Molecular Anthropology, Centre for Genome Biology Department of Biological, Geological and Environmental Sciences (BiGeA), University of Bologna, via Selmi 3, Bologna 40126, Italy.
Department of Agricultural Sciences, University of Bologna, viale Fanin 42, Bologna 40127, Italy.
Nutrients. 2016 Oct 22;8(10):660. doi: 10.3390/nu8100660.
Coeliac disease (CD) is associated with alterations of the intestinal microbiota. Although several strains showed anti-inflammatory activity and prevention of toxic gliadin peptides generation in vitro, few data are available on their efficacy when administered to CD subjects. This study evaluated the effect of administration for three months of a food supplement based on two strains (B632 and BR03) to restore the gut microbial balance in coeliac children on a gluten free diet (GFD). Microbial DNA was extracted from faeces of 40 coeliac children before and after probiotic or placebo administration and 16 healthy children (Control group). Sequencing of the amplified V3-V4 hypervariable region of 16S rRNA gene as well as qPCR of spp., spp., group and enterobacteria were performed. The comparison between CD subjects and Control group revealed an alteration in the intestinal microbial composition of coeliacs mainly characterized by a reduction of the ratio, of and . Regarding the effects of the probiotic, an increase of was found as well as a re-establishment of the physiological ratio. Therefore, a three-month administration of strains helps in restoring the healthy percentage of main microbial components.
乳糜泻（CD）与肠道微生物群的改变有关。尽管有几种菌株在体外显示出抗炎活性并能预防毒性麦醇溶蛋白肽的产生，但关于将其应用于CD患者时的疗效，相关数据却很少。本研究评估了基于两种菌株（B632和BR03）的食品补充剂连续三个月给药对恢复无麸质饮食（GFD）的乳糜泻儿童肠道微生物平衡的影响。在益生菌或安慰剂给药前后，从40名乳糜泻儿童以及16名健康儿童（对照组）的粪便中提取微生物DNA。对16S rRNA基因的V3-V4高变区进行扩增测序，并对双歧杆菌属、乳杆菌属、拟杆菌群和肠杆菌进行定量聚合酶链反应（qPCR）。CD患者与对照组之间的比较显示，乳糜泻患者肠道微生物组成发生改变，主要表现为双歧杆菌与拟杆菌的比例、厚壁菌门和放线菌门减少。关于益生菌的作用，发现双歧杆菌增加，并且生理上的双歧杆菌与拟杆菌比例得以重建。因此，连续三个月施用这两种菌株有助于恢复主要微生物成分的健康比例。
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