通过重组人转化生长因子-β在牙周组织再生中的成骨和成牙作用：食蟹猴的初步研究。
Cementogenesis and osteogenesis in periodontal tissue regeneration by recombinant human transforming growth factor-β : a pilot study in Papio ursinus.
机构信息
Bone Research Laboratory, Department of Oral Medicine & Periodontology, School of Oral Health Sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, Johannesburg, South Africa.
Department of Oral Biological Sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, Johannesburg, South Africa.
出版信息
J Clin Periodontol. 2017 Jan;44(1):83-95. doi: 10.1111/jcpe.12642. Epub 2016 Dec 1.
OBJECTIVES
The aim of this study was to investigate cementogenesis and alveolar bone induction during in vivo periodontal tissue regeneration upon implantation of hTGF-β in furcation defects of Papio ursinus and to evaluate the feasibility of gene expression studies.
MATERIALS AND METHODS
Class II furcation defects (day 0) were prepared in mandibular first and second molars of three P. ursinus and on day 30 implanted with and without 75 μg hTGF-β in Matrigel matrix. On day 0, 30 and 90, cementum and alveolar bone were harvested for gene expression analyses. Coral-derived bioreactors with and without 250 μg hTGF-β were implanted in the rectus abdominis to monitor tissue induction.
RESULTS
hTGF-β induced cementogenesis with TGF-β , Cementum Protein-1 (Cemp1) and Osteocalcin (OC) up-regulation, and down-regulation of BMP-2 and OP-1. Matrigel matrix specimens showed up-regulation of BMP-2, TGF-β , and OC, with down-regulation of OP-1 and Cemp1. hTGF-β induced alveolar bone with down-regulation of OP-1, TGF-β , OC, and Cemp1. hTGF-β bioreactors induced bone at the periphery only. BMP-3, BMP-4, TGF-β and TGF-β were up-regulated in the adjacent muscle with TGF-β down-regulation.
CONCLUSIONS
Cementogenesis and osteogenesis by hTGF-β entail the expression and up-regulation of TGF-β and OC with fine tuning and modulation of BMP-2 and OP-1.
目的
本研究旨在探讨 hTGF-β 在狒狒下颌第一、二磨牙分叉缺损模型中的植入对体内牙周组织再生过程中牙骨质形成和牙槽骨诱导的影响，并评估基因表达研究的可行性。
材料与方法
在 3 只狒狒下颌第一、二磨牙的分叉缺损（第 0 天）中制备 II 类分叉缺损，并在第 30 天植入含或不含 75μg hTGF-β 的 Matrigel 基质。在第 0、30 和 90 天，采集牙骨质和牙槽骨用于基因表达分析。在有无 250μg hTGF-β 的珊瑚衍生生物反应器中植入腹直肌，以监测组织诱导。
结果
hTGF-β 通过上调 TGF-β、牙骨质蛋白 1（Cemp1）和骨钙素（OC），下调 BMP-2 和 OP-1 诱导牙骨质形成。Matrigel 基质标本显示 BMP-2、TGF-β 和 OC 上调，OP-1 和 Cemp1 下调。hTGF-β 通过下调 OP-1、TGF-β、OC 和 Cemp1 诱导牙槽骨形成。hTGF-β 生物反应器仅在边缘诱导骨形成。BMP-3、BMP-4、TGF-β 和 TGF-β 在相邻肌肉中上调，而 TGF-β 下调。
结论
hTGF-β 诱导的牙骨质和骨形成涉及 TGF-β 和 OC 的表达和上调，同时精细调节 BMP-2 和 OP-1。
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