从初始前体细胞分化为辅助性T细胞亚群的策略。

Strategies for T Helper Cell Subset Differentiation from Naïve Precursors.

作者信息

Annunziato Francesco, Maggi Laura

机构信息

Department of Experimental and Clinical Medicine, University of Florence and Regenerative Medicine Unit, Careggi University Hospital Florence, Viale Pieraccini 6, Florence, 50134, Italy.

出版信息

Methods Mol Biol. 2017;1514:127-137. doi: 10.1007/978-1-4939-6548-9_11.

DOI:10.1007/978-1-4939-6548-9_11

PMID:27787798

Abstract

In vitro strategies to obtain different T helper cell subsets from naïve precursors are usually characterized by anti-CD3 plus anti-CD28 monoclonal antibody stimulation of naïve CD4+ T cells (derived from both peripheral blood or umbilical cord blood) in the presence of specific polarizing cytokines. In this chapter, we describe detailed protocols to obtain in vitro differentiation of human naïve CD4+ T cells into Th1, Th2, or Th17 cells.

摘要

从初始前体细胞获得不同辅助性T细胞亚群的体外策略通常具有以下特点：在特定极化细胞因子存在的情况下，用抗CD3加抗CD28单克隆抗体刺激初始CD4 + T细胞（来源于外周血或脐带血）。在本章中，我们描述了将人初始CD4 + T细胞体外分化为Th1、Th2或Th17细胞的详细方案。