Karpov Dmitry S, Grineva Evgenia N, Leinsoo Arvo T, Nadolinskaia Nonna I, Danilenko Nataliya K, Tutyaeva Vera V, Spasskaya Daria S, Preobrazhenskaya Olga V, Lysov Yuriy P, Karpov Vadim L
Engelhardt Institute of Molecular Biology Russian Academy of Sciences, Department of Intracellular proteolysis regulation, Vavilov str. 32 Moscow 119991, Russia.
Orekhovich Institute of Biomedical Chemistry, Laboratory of Medicinal Proteomics, Pogodinskaya str. 10 Moscow, 119121, Russia.
FEMS Yeast Res. 2017 Jan 1;17(1). doi: 10.1093/femsyr/fow098.
The transcription factor ScRpn4 coordinates the expression of Saccharomyces cerevisiae proteasomal genes. ScRpn4 orthologues are found in a number of other Saccharomycetes yeasts. Their functions, however, have not yet been characterised experimentally in vivo . We expressed the Debaryomyces hansenii DEHA2D12848 gene encoding an ScRpn4 orthologue (DhRpn4), in an S. cerevisiae strain lacking RPN4 . We showed that DhRpn4 activates transcription of proteasomal genes using ScRpn4 binding site and provides resistance to various stresses. The 43-238 aa segment of DhRpn4 contains an unique portable transactivation domain. Similar to the ScRpn4 N-terminus, this domain lacks a compact structure Moreover, upon overexpression in D. hansenii , DhRpn4 upregulates protesomal genes. Thus, we show that DhRpn4 is the activator for proteasomal genes.
转录因子ScRpn4协调酿酒酵母蛋白酶体基因的表达。在许多其他酵母属酵母中发现了ScRpn4的直系同源物。然而，它们的功能尚未在体内进行实验表征。我们在缺乏RPN4的酿酒酵母菌株中表达了编码ScRpn4直系同源物（DhRpn4）的汉逊德巴利酵母DEHA2D12848基因。我们表明，DhRpn4利用ScRpn4结合位点激活蛋白酶体基因的转录，并提供对各种应激的抗性。DhRpn4的43 - 238氨基酸片段包含一个独特的可移植反式激活结构域。与ScRpn4的N端相似，该结构域缺乏紧密结构。此外，在汉逊德巴利酵母中过表达时，DhRpn4上调蛋白酶体基因。因此，我们表明DhRpn4是蛋白酶体基因的激活剂。
Zotero 插件