从一名携带新型纯合PAI-1突变患者的尿液来源细胞中生成人诱导多能干细胞。
Generation of human iPSCs from urine derived cells of a patient with a novel homozygous PAI-1 mutation.
作者信息
Afzal Muhammad Zeeshan, Gartz Melanie, Klyachko Ekaterina A, Khan Sadiya Sana, Shah Sanjiv J, Gupta Sweta, Shapiro Amy D, Vaughan Douglas E, Strande Jennifer L
机构信息
Department of Medicine and Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI, United States.
Department of Medicine, Northwestern University-Feinberg School of Medicine, Chicago, IL, United States.
出版信息
Stem Cell Res. 2016 Nov;17(3):657-660. doi: 10.1016/j.scr.2016.11.010. Epub 2016 Nov 9.
Abstract
We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from subject with a novel homozygous Plasminogen Activator Inhibitor-1 (PAI-1 null) mutation. The Sendai virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the PAI-1 UCs.
摘要
我们使用从患有新型纯合纤溶酶原激活物抑制剂-1（PAI-1基因缺失）突变的受试者收集的尿液来源细胞（UCs），在无饲养层培养条件下生成了一条人诱导多能干细胞（iPSC）系。编码多能性山中转录因子的仙台病毒（SeV）载体以低感染复数用于重编程PAI-1 UCs。
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