植物乳杆菌 ST8Sh 有益特性的体外评价
In Vitro Evaluation of Beneficial Properties of Bacteriocinogenic Lactobacillus plantarum ST8Sh.
机构信息
Veterinary Department, Universidade Federal de Viçosa, Campus UFV, Viçosa, 36570-900, Minas Gerais, Brazil.
AGEE, Handong Global University, Pohang, South Korea.
出版信息
Probiotics Antimicrob Proteins. 2017 Jun;9(2):194-203. doi: 10.1007/s12602-016-9245-7.
Lactobacillus plantarum ST8Sh, isolated from Bulgarian salami "shpek" and previously characterized as bacteriocin producer, was evaluated for its beneficial properties. Based on the PCR analysis, Lb. plantarum ST8Sh was shown to host a gene related to the production of adhesion proteins such as Mab, Mub, EF, and PrgB. Genetic and physiological tests suggest Lb. plantarum ST8Sh to represent a potential probiotic candidate, including survival in the presence of low levels of pH and high levels of ox bile, production of β-galactosidase, bile salt deconjugation, high level of hydrophobicity, functional auto- and co-aggregation properties, and adhesion to cell lines. Application of semi-purified bacteriocin produced by Lb. plantarum ST8Sh in combination with ciprofloxacin presented synergistic effect on inhibition of Listeria monocytogenes Scott A. Based on observed properties, Lb. plantarum ST8Sh can be considered as a potential probiotic candidate with additional bacteriocinogenic properties.
植物乳杆菌 ST8Sh 从保加利亚香肠“shpek”中分离出来，先前被鉴定为细菌素产生菌，其有益特性得到了评估。基于 PCR 分析，发现植物乳杆菌 ST8Sh 携带与黏附蛋白（如 Mab、Mub、EF 和 PrgB）产生相关的基因。遗传和生理测试表明，植物乳杆菌 ST8Sh 可能是一种潜在的益生菌候选菌株，包括在低 pH 值和高胆盐浓度下的生存能力、β-半乳糖苷酶的产生、胆盐去结合、高水平的疏水性、功能自聚集和共聚聚集特性，以及对细胞系的黏附性。应用植物乳杆菌 ST8Sh 半纯化细菌素与环丙沙星联合应用对李斯特菌 Scott A 表现出协同抑制作用。基于观察到的特性，植物乳杆菌 ST8Sh 可以被认为是一种具有额外细菌素产生特性的潜在益生菌候选菌株。
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