白藜芦醇对糖尿病大鼠脂肪组织抵抗素和apelin 基因表达的影响。

Effect of resveratrol on resistin and apelin gene expressions in adipose tissue of diabetic rats.

机构信息

Department of Clinical Biochemistry, Medical School, Hamadan University of Medical Sciences, Hamadan, Iran.

Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.

出版信息

Turk J Med Sci. 2016 Nov 17;46(5):1561-1567. doi: 10.3906/sag-1505-6.

DOI:10.3906/sag-1505-6

PMID:27966329

Abstract

BACKGROUND/AIM: Adipose tissue plays a major role in glucose homeostasis. Dietary antioxidants such as resveratrol (RSV) may offer some protection against the early stage of diabetes mellitus and the development of complications. The present study investigated the effects of RSV on biochemical parameters and resistin and apelin gene expressions in adipose tissue of rats with type 2 diabetes.

MATERIALS AND METHODS

Diabetes was induced using a single dose of streptozotocin + nicotinamide. Three groups of diabetic rats were treated with different doses of RSV (1, 5, and 10 mg/kg body weight per day). Oxidative status, serum biochemical parameters, insulin, and HOMA index were measured. Finally, resistin and apelin gene expressions were determined in rats' adipose tissue using RT- PCR (qRT-PCR).

RESULTS

A significant reduction in serum glucose level was observed in rats treated with 5 and 10 mg/kg per day RSV compared with the diabetic control. Resistin expression in adipose tissue was reduced in RSV-treated groups, while no significant changes were observed in apelin expression.

CONCLUSION

The results showed that RSV improves insulin sensitivity due to a simultaneous decrease in blood glucose and an increase in insulin. We concluded that RSV has potential hypoglycemic effect, probably by increasing insulin levels and changing the expression of resistin.

摘要

背景/目的：脂肪组织在葡萄糖稳态中起着重要作用。膳食抗氧化剂，如白藜芦醇（RSV），可能对糖尿病早期和并发症的发展提供一定的保护。本研究旨在探讨 RSV 对 2 型糖尿病大鼠生化参数和脂肪组织抵抗素和 Apelin 基因表达的影响。