使用异种移植小鼠模型的共聚焦/双光子显微镜技术研究癌细胞在骨中的定植情况。
Confocal/two-photon microscopy in studying colonisation of cancer cells in bone using xenograft mouse models.
作者信息
Allocca Gloria, Kusumbe Anjali P, Ramasamy Saravana K, Wang Ning
机构信息
Department of Oncology and Metabolism, The Mellanby Centre for Bone Research, The University of Sheffield , Sheffield, UK.
Kennedy Institute of Rheumatology, University of Oxford , Oxford, UK.
出版信息
Bonekey Rep. 2016 Dec 7;5:851. doi: 10.1038/bonekey.2016.84. eCollection 2016.
Abstract
Confocal and two-photon microscopy has been widely used in bone research to not only produce high quality, three-dimensional images but also to provide valuable structural and quantitative information. In this article, we describe step-by-step protocols for confocal and two-photon microscopy to investigate earlier cellular events during colonisation of cancer cells in bone using xenograft mouse models. This includes confocal/two-photon microscopy imaging of paraformaldehyde fixed thick bone sections and frozen bone samples.
摘要
共聚焦显微镜和双光子显微镜已广泛应用于骨研究，不仅能生成高质量的三维图像，还能提供有价值的结构和定量信息。在本文中，我们描述了使用异种移植小鼠模型，通过共聚焦显微镜和双光子显微镜研究癌细胞在骨中定植过程中早期细胞事件的分步方案。这包括对多聚甲醛固定的厚骨切片和冷冻骨样本进行共聚焦/双光子显微镜成像。
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本文引用的文献
1
Dormant breast cancer micrometastases reside in specific bone marrow niches that regulate their transit to and from bone.
Sci Transl Med. 2016 May 25;8(340):340ra73. doi: 10.1126/scitranslmed.aad4059.
2
Age-dependent modulation of vascular niches for haematopoietic stem cells.
Nature. 2016 Apr 21;532(7599):380-4. doi: 10.1038/nature17638. Epub 2016 Apr 13.
3
Mouse models for studying prostate cancer bone metastasis.
Bonekey Rep. 2016 Feb 17;5:777. doi: 10.1038/bonekey.2016.4. eCollection 2016.
4
An Improved Immunostaining and Imaging Methodology to Determine Cell and Protein Distributions within the Bone Environment.
J Histochem Cytochem. 2016 Mar;64(3):168-78. doi: 10.1369/0022155415626765. Epub 2015 Dec 30.
5
Osteoclasts control reactivation of dormant myeloma cells by remodelling the endosteal niche.
Nat Commun. 2015 Dec 3;6:8983. doi: 10.1038/ncomms9983.
6
Sample preparation for high-resolution 3D confocal imaging of mouse skeletal tissue.
Nat Protoc. 2015 Dec;10(12):1904-14. doi: 10.1038/nprot.2015.125. Epub 2015 Oct 29.
7
The frequency of osteolytic bone metastasis is determined by conditions of the soil, not the number of seeds; evidence from in vivo models of breast and prostate cancer.
J Exp Clin Cancer Res. 2015 Oct 20;34:124. doi: 10.1186/s13046-015-0240-8.
8
Mitotic quiescence, but not unique "stemness," marks the phenotype of bone metastasis-initiating cells in prostate cancer.
FASEB J. 2015 Aug;29(8):3141-50. doi: 10.1096/fj.14-266379. Epub 2015 Apr 17.
9
Intravital bone imaging by two-photon excitation microscopy to identify osteocytic osteolysis in vivo.
Bone. 2015 May;74:134-9. doi: 10.1016/j.bone.2015.01.013. Epub 2015 Jan 24.
10
Prostate cancer cells preferentially home to osteoblast-rich areas in the early stages of bone metastasis: evidence from in vivo models.
J Bone Miner Res. 2014 Dec;29(12):2688-96. doi: 10.1002/jbmr.2300.
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