鲽形目鱼类的着丝粒卫星DNA及其与物种形成过程的关系。

Centromeric Satellite DNA in Flatfish (Order Pleuronectiformes) and Its Relation to Speciation Processes.

作者信息

Rodríguez Francisca Robles, de la Herrán Roberto, Navajas-Pérez Rafael, Cano-Roldán Belén, Sola-Campoy Pedro Juan, García-Zea Jerson Alexander, Rejón Carmelo Ruiz

机构信息

From the Departamento de Genética, Facultad de Ciencias, Universidad de Granada, Avda. Fuentenueva s/n, 18071 Granada, Spain.

出版信息

J Hered. 2017 Mar 1;108(2):217-222. doi: 10.1093/jhered/esw076.

DOI:10.1093/jhered/esw076

PMID:28173078

Abstract

Two new centromeric satellite DNAs in flatfish (Order Pleuronectiformes) have been characterized. The SacI-family from Hippoglossus hippoglossus, restricted to this species, had a monomeric size of 334 base pair (bp) and was located in most of the centromeres of its karyotype. The PvuII-family, with a monomeric size of 177 bp, was initially isolated from the genome of Solea senegalensis, and fluorescent in situ hybridization (FISH) localized the repeat to centromeres of most of the chromosomes. This family could only be amplified in 2 other species of the genus Solea (Solea solea and Solea lascaris). Molecular features and chromosomal location indicated a possible structural and/or functional role of these sequence repeats. The presence of species-specific satellite-DNA families in the centromeres and their possible role in the speciation processes in this group of fishes is discussed.

摘要

已对鲽形目（Pleuronectiformes）鱼类中的两种新的着丝粒卫星DNA进行了表征。庸鲽（Hippoglossus hippoglossus）的SacI家族仅限于该物种，其单体大小为334碱基对（bp），位于其核型的大多数着丝粒中。PvuII家族的单体大小为177 bp，最初是从塞内加尔鳎（Solea senegalensis）的基因组中分离出来的，荧光原位杂交（FISH）将该重复序列定位到大多数染色体的着丝粒上。这个家族只能在鳎属（Solea）的另外两个物种（欧洲鳎（Solea solea）和拉斯卡里斯鳎（Solea lascaris））中扩增。分子特征和染色体定位表明这些序列重复可能具有结构和/或功能作用。讨论了着丝粒中物种特异性卫星DNA家族的存在及其在这组鱼类物种形成过程中的可能作用。