Zhang Dexian, Zhao Jingcui, Wang Qiuxia, Liu Yaochuan, Tian Chunlian, Zhao Yujun, Yu Lihui, Liu Mingchun
Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, PR China.
Jianping Livestock Product Safety Supervision, Chaoyang 122400, PR China.
Microb Pathog. 2017 Apr;105:51-56. doi: 10.1016/j.micpath.2017.02.010. Epub 2017 Feb 8.
Trueperella pyogenes plays a crucial role in endometritis pathogenesis and is also associated with many infections, including metritis, mastitis, arthritis and liver abscessation, in many domestic animals. In this study, we investigated the prevalence of tetracycline resistance in T. pyogenes isolated from dairy cows with endometritis in Inner Mongolia, China, and we assessed tetracycline-resistance gene distribution among the isolates. Our results indicated that 68.7% and 62.5% of the isolates were resistant to tetracycline and doxycycline, respectively, and the rate of resistance to metacycline was 18.8%. The tetracycline resistance gene tetK was present in all isolates (n = 32), whereas the tetM gene was identified in 12.5% and 9.4% of the isolates, in the chromosome and plasmid, respectively. Strains carrying tetW were also common in the chromosome and plasmid, with abundances of 53.1% and 46.9%, respectively. However, tetO and otrA were absent in all isolates. The resistance phenotype analysis indicated that 6.3% of strains were susceptible to all tetracyclines, while 3.1% showed resistance to all tetracyclines.
化脓放线杆菌在子宫内膜炎发病机制中起关键作用，在许多家畜中还与包括子宫炎、乳腺炎、关节炎和肝脓肿在内的多种感染有关。在本研究中，我们调查了从中国内蒙古患子宫内膜炎的奶牛中分离出的化脓放线杆菌对四环素的耐药率，并评估了分离株中四环素耐药基因的分布情况。我们的结果表明，分别有68.7%和62.5%的分离株对四环素和强力霉素耐药，对美他环素的耐药率为18.8%。所有分离株（n = 32）均存在四环素耐药基因tetK，而tetM基因分别在12.5%和9.4%的分离株的染色体和质粒中被鉴定出。携带tetW的菌株在染色体和质粒中也很常见，丰度分别为53.1%和46.9%。然而，所有分离株中均未检测到tetO和otrA。耐药表型分析表明，6.3%的菌株对所有四环素敏感，而3.1%的菌株对所有四环素耐药。
