Xing Dongxu, Yang Qiong, Jiang Liang, Li Qingrong, Xiao Yang, Ye Mingqiang, Xia Qingyou
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China.
Sericulture and Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China.
Int J Mol Sci. 2017 Feb 10;18(2):234. doi: 10.3390/ijms18020234.
The silkworm is an economically important species. White muscardine caused by is the main fungal disease in sericulture, and understanding the silkworm responses to infection is of particular interest. Herein, we investigated the molecular mechanisms underlying these responses in two silkworm strains Haoyue (HY, sensitive to ) and Kang 8 (K8, resistant to ) using an RNA-seq approach. For each strain, three biological replicates for immersion treatment, two replicates for injection treatment and three untreated controls were collected to generate 16 libraries for sequencing. Differentially expressed genes (DEGs) between treated samples and untreated controls, and between the two silkworm strains, were identified. DEGs and the enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways of the two strains exhibited an obvious difference. Several genes encoding cuticle proteins, serine proteinase inhibitors (SPI) and antimicrobial peptides (AMP) and the drug metabolism pathway involved in toxin detoxification were considered to be related to the resistance of K8 to These results revealed insight into the resistance and susceptibility of two silkworm strains against infection and provided a roadmap for silkworm molecular breeding to enhance its resistance to .
家蚕是一种具有重要经济价值的物种。由[病原菌名称缺失]引起的白僵病是养蚕业中的主要真菌病害，了解家蚕对[病原菌名称缺失]感染的反应尤为重要。在此，我们使用RNA测序方法研究了两个家蚕品系皓月（HY，对[病原菌名称缺失]敏感）和康8（K8，对[病原菌名称缺失]有抗性）对这些反应的分子机制。对于每个品系，收集了浸浴处理的三个生物学重复、注射处理的两个重复以及三个未处理的对照，以生成16个文库进行测序。鉴定了处理样品与未处理对照之间以及两个家蚕品系之间的差异表达基因（DEG）。两个品系的DEG和富集京都基因与基因组百科全书（KEGG）通路表现出明显差异。几个编码表皮蛋白、丝氨酸蛋白酶抑制剂（SPI）和抗菌肽（AMP）的基因以及参与毒素解毒的药物代谢途径被认为与K8对[病原菌名称缺失]的抗性有关。这些结果揭示了两个家蚕品系对[病原菌名称缺失]感染的抗性和易感性，并为家蚕分子育种提高其对[病原菌名称缺失]的抗性提供了路线图。
