犬粪便中二元毒素和A/B毒素的分离及分子检测
Isolation of and molecular detection of binary and A/B toxins in faeces of dogs.
作者信息
Ghavidel M, Salari Sedigh H, Razmyar J
机构信息
Ph.D. Student in Bacteriology, Department of Microbiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
出版信息
Iran J Vet Res. 2016 Fall;17(4):273-276.
The aim of this study was to isolate from dogs' faeces, and to study the frequency of its virulence genes. A total of 151 samples of dogs' faeces were collected. The isolation of was performed by using the bacterial culture methods followed by DNA extraction using boiling method. Multiplex PCR method was performed for identification of , , and genes and single method was carried out for detection of . Twelve samples (7.9%) were positive in bacteriological assay and based on molecular assay, 66.7% of the isolates (8 of 12 isolated) had shown tcdA, tcdB profile. This is the first investigation on molecular assay of in Iran's dog population.
本研究的目的是从犬粪便中分离出[具体细菌名称未给出]，并研究其毒力基因的频率。共收集了151份犬粪便样本。通过细菌培养方法进行[具体细菌名称未给出]的分离，随后采用煮沸法进行DNA提取。采用多重PCR方法鉴定[具体细菌名称未给出]的[具体基因名称未给出]、[具体基因名称未给出]、[具体基因名称未给出]和[具体基因名称未给出]基因，采用单一方法检测[具体基因名称未给出]。12份样本（7.9%）细菌学检测呈阳性，基于分子检测，66.7%的分离株（12株分离株中的8株）显示出tcdA、tcdB谱型。这是伊朗犬类群体中对[具体细菌名称未给出]进行分子检测的首次调查。
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