Pan Fengshan, Luo Sha, Shen Jing, Wang Qiong, Ye Jiayuan, Meng Qian, Wu Yingjie, Chen Bao, Cao Xuerui, Yang Xiaoe, Feng Ying
MOE Key Laboratory of Environment Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, People's Republic of China.
Agricultural Technology Extension Center of Shaoxing, Shaoxing, 312099, Zhejiang Province, People's Republic of China.
Environ Sci Pollut Res Int. 2017 Apr;24(10):9350-9360. doi: 10.1007/s11356-017-8565-9. Epub 2017 Feb 23.
A hydroponic experiment was conducted to investigate the effects of an endophytic bacterium SaMR12 on Sedum alfredii Hance metal ion accumulation, chlorophyll concentration, and the expression of three metal transporter families, zinc-regulated transporters, iron-regulated transporter-like protein (ZIP); natural resistance-associated macrophage protein; and heavy metal ATPase (HMA) at different Cd treatment levels. The results showed that at relatively low Cd conditions (≤25 μM), SaMR12 demonstrated a 19.5-27.5% increase in Fe, a 46.7-90.7% increase in Zn, and a 7.9-43.7% increase in Cu content in the shoot and elevated expression of SaIRT1, SaZIP3, SaHMA2, and SaNramp3 in the shoot and SaZIP1, SaHMA2, SaNramp1, and SaNramp3 in the root. At high Cd conditions (100 and 400 μM), SaMR12 demonstrated a 16.4-18.5% increase in leaf chlorophyll concentration, a 18.9-23.2% increase in Fe, and a 15.4-17.5% increase in Mg content in the shoot and elevated expression of SaZIP3, SaNramp6, SaHMA2, and SaHMA3 in the shoot and SaZIP3, SaNarmp1, SaNarmp3, and SaNarmp6 in the root. These results indicated that SaMR12 can elevate essential metal ion uptake and regulate the expression of transport genes to promote plant growth and enhance Cd tolerance and uptake to improve Cd accumulation up to 118-130%.
进行了一项水培实验，以研究内生细菌SaMR12在不同镉处理水平下对东南景天金属离子积累、叶绿素浓度以及三个金属转运蛋白家族（锌调节转运蛋白、铁调节转运蛋白样蛋白（ZIP）；天然抗性相关巨噬蛋白；重金属ATP酶（HMA））表达的影响。结果表明，在相对较低的镉条件下（≤25 μM），SaMR12使地上部铁含量增加19.5 - 27.5%，锌含量增加46.7 - 90.7%，铜含量增加7.9 - 43.7%，并使地上部SaIRT1、SaZIP3、SaHMA2和SaNramp3以及根部SaZIP1、SaHMA2、SaNramp1和SaNramp3的表达升高。在高镉条件下（100和400 μM），SaMR12使地上部叶片叶绿素浓度增加16.4 - 18.5%，铁含量增加18.9 - 23.2%，镁含量增加15.4 - 17.5%，并使地上部SaZIP3、SaNramp6、SaHMA2和SaHMA3以及根部SaZIP3、SaNarmp1、SaNarmp3和SaNarmp6的表达升高。这些结果表明，SaMR12可以提高必需金属离子的吸收并调节转运基因的表达，以促进植物生长并增强镉耐受性和吸收，使镉积累提高118 - 130%。
