孕酮需要人类精子中的热休克蛋白90（HSP90）来调节运动和顶体反应。
Progesterone requires heat shock protein 90 (HSP90) in human sperm to regulate motility and acrosome reaction.
作者信息
Sagare-Patil Vrushali, Bhilawadikar Rashmi, Galvankar Mosami, Zaveri Kusum, Hinduja Indira, Modi Deepak
机构信息
Molecular and Cellular Biology Laboratory, National Institute for Research in Reproductive Health (ICMR), J. M. Street, Parel, Mumbai, 400012, India.
Hinduja IVF Centre, PD Hinduja Hospital and Medical Research Center, Veer Savarkar Marg, Mahim, Mumbai, 400016, India.
出版信息
J Assist Reprod Genet. 2017 Apr;34(4):495-503. doi: 10.1007/s10815-017-0879-5. Epub 2017 Feb 24.
PURPOSE
The aims of this paper were to study whether heat shock protein 90 (HSP90) is a regulator of sperm functions and to determine its association with oligoasthenozoospermia.
METHODS
The levels of HSP90 in sperm lysates were measured by ELISA. Localization of HSP90 and its isoforms was evaluated by immunofluorescence. Sperm motility and kinetics were assessed by computer-assisted sperm analysis. Acrosome reaction was determined by lectin staining.
RESULTS
The levels of HSP90 were lower in oligoasthenozoospermic men and correlated positively with the number of motile spermatozoa. In capacitated human spermatozoa, HSP90α was mostly found in residual nuclear envelope, and the HSP90β isoform was higher in the flagella. Inhibition of HSP90 by geldanamycin or 17-AAG did not affect basal motility, but suppressed progesterone-mediated forward progressive motility, hyperactivation and acrosome reaction. Progesterone treatment dephosphorylated both HSP90α and HSP90β at Ser/Thr-Pro residues, but not Tyr residues.
CONCLUSION
HSP90 levels are downregulated in oligoasthenozoospermia, and its functional inhibition attenuates progesterone-mediated sperm motility and acrosome reaction.
目的
本文旨在研究热休克蛋白90（HSP90）是否为精子功能的调节因子，并确定其与少弱精子症的关联。
方法
采用酶联免疫吸附测定法（ELISA）检测精子裂解物中HSP90的水平。通过免疫荧光评估HSP90及其异构体的定位。利用计算机辅助精子分析评估精子活力和动力学。通过凝集素染色确定顶体反应。
结果
少弱精子症男性的HSP90水平较低，且与活动精子数量呈正相关。在获能的人类精子中，HSP90α主要存在于残留核膜中，而HSP90β异构体在鞭毛中含量较高。格尔德霉素或17-AAG对HSP90的抑制不影响基础活力，但抑制了孕酮介导的前向渐进性活力、超活化和顶体反应。孕酮处理使HSP90α和HSP90β在丝氨酸/苏氨酸-脯氨酸残基处去磷酸化，但在酪氨酸残基处未去磷酸化。
结论
少弱精子症中HSP90水平下调，其功能抑制减弱了孕酮介导的精子活力和顶体反应。
Zotero 插件