一种用于在芯片上对人类卵母细胞传输特性进行分析的微流控灌注方法。
A microfluidic perfusion approach for on-chip characterization of the transport properties of human oocytes.
机构信息
Department of Electronic Science and Technology, University of Science and Technology of China, Hefei, Anhui 230027, China.
Reproductive Medicine Center, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China.
出版信息
Lab Chip. 2017 Mar 29;17(7):1297-1305. doi: 10.1039/c6lc01532h.
Accurate characterization of the cell membrane transport properties of human oocytes is of great significance to reproductive pharmacology, fertility preservation, and assisted reproduction. However, the commonly used manual method for quantifying the transport properties is associated with uncontrolled operator-to-operator and run-to-run variability. Here, we report a novel sandwich structured microfluidic device that can be readily fabricated for characterizing oocyte membrane transport properties. Owing to its capacity for excellent control of both solution replacement and temperature in the microchannel, the temperature-dependent permeability of the oocyte membrane can be precisely characterized. Furthermore, the fertilization and developmental competence analysis post perfusion indicate that our approach does not compromise the physiological function of in vitro matured human oocytes. Collectively, we present the development of a novel sandwich structured microfluidic device based approach that allows on-chip characterization of the transport properties of human oocytes under innocuous osmotic shock or injury to the cells.
准确描述人卵细胞膜转运特性对于生殖药理学、生育力保存和辅助生殖具有重要意义。然而，常用的手动方法定量测定转运特性与不可控的操作者间和运行间变异性相关。在这里，我们报告了一种新型的三明治结构微流控装置，可用于表征卵细胞膜转运特性。由于其在微通道中对溶液替换和温度具有出色的控制能力，因此可以精确表征卵细胞膜的温度依赖性通透性。此外，灌注后的受精和发育能力分析表明，我们的方法不会损害体外成熟人卵的生理功能。总的来说，我们提出了一种基于新型三明治结构微流控装置的方法，该方法可在对细胞无伤害的渗透冲击下或损伤下，实现人卵转运特性的芯片上表征。
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