生理离子强度和温度下的血红蛋白-膜相互作用

Hemoglobin-membrane interaction at physiological ionic strength and temperature.

作者信息

Lilley G L, Fung L W

机构信息

Department of Chemistry, Loyola University of Chicago, IL 60626.

出版信息

Life Sci. 1987 Nov 30;41(22):2429-36. doi: 10.1016/0024-3205(87)90668-0.

DOI:10.1016/0024-3205(87)90668-0

PMID:2824953

Abstract

The spin-label electron paramagnetic resonance (EPR) technique has been used to study the interaction between human hemoglobin and erythrocyte membranes as a function of temperature and ionic strength. We show, for the first time, experimental evidence for the existence of the interaction at physiological pH, ionic strength and temperature. In addition to the pH dependence that we have previously reported, the interactions are also temperature and ionic strength dependent. Using a simple two-state equilibrium model to analyze the EPR data, we obtain an equilibrium dissociation constant of about 8.1 +/- 5.6 X 10(-5) M for hemoglobin-membrane systems in 5 mM phosphate with 150 mM NaCl at pH 7.4 and 37 degrees C.

摘要

自旋标记电子顺磁共振（EPR）技术已被用于研究人血红蛋白与红细胞膜之间的相互作用，该相互作用是温度和离子强度的函数。我们首次展示了在生理pH值、离子强度和温度下存在这种相互作用的实验证据。除了我们之前报道的pH依赖性外，这些相互作用还依赖于温度和离子强度。使用简单的二态平衡模型分析EPR数据，对于在pH 7.4和37摄氏度下含有150 mM氯化钠的5 mM磷酸盐缓冲液中的血红蛋白-膜系统，我们得到的平衡解离常数约为8.1 +/- 5.6 X 10(-5) M。