Luo Ping, Lu Gaixia, Fan Lin-Lin, Zhong Xiaoming, Yang Huiqiong, Xie Ruting, Lv Zhongwei, Lv Qian-Zhou, Fu Da, Yang Li-Xin, Ma Yushui
Department of Breast Cancer, Nanchang Third Hospital, Nanchang, Jiangxi 330002, P.R. China.
Department of Nuclear Medicine, Shanghai 10th People's Hospital, Tongji University School of Medicine, Shanghai 200072, P.R. China.
Oncol Rep. 2017 Apr;37(4):2057-2062. doi: 10.3892/or.2017.5449. Epub 2017 Feb 14.
The present study was carried out to investigate the clinical significance of TMPRSS3 and TNFRSF11B in breast cancer. Thus, the expression levels of TMPRSS3 and TNFRSF11B and the correlation with prognosis in patients with breast cancer were analyzed in silico using gene microarray and hierarchical clustering analysis. Then, the differential expression in breast cancer vs. normal breast tissue was explored in the Oncomine platform and verified in our independent samples using IHC technique. Our results indicated that TMPRSS3 was upregulated and TNFRSF11B was downregulated in breast cancer tissues compared with the levels in the human normal breast tissues. TMPRSS3 and TNFRSF11B were confirmed to be correlated with distant organ metastasis of breast cancer. Moreover, upregulation of TMPRSS3 accompanied by downregulation of TNFRSF11B was found to be associated with a shorter median overall survival and indicated a poor prognosis. In conclusion, TMPRSS3 and TNFRSF11B may have potential prognostic value to be used as tumor biomarkers in breast cancer patients.
本研究旨在探讨跨膜丝氨酸蛋白酶3（TMPRSS3）和肿瘤坏死因子受体超家族成员11B（TNFRSF11B）在乳腺癌中的临床意义。因此，利用基因芯片和层次聚类分析，在计算机上分析了乳腺癌患者中TMPRSS3和TNFRSF11B的表达水平及其与预后的相关性。然后，在Oncomine平台上探索了乳腺癌组织与正常乳腺组织中的差异表达，并使用免疫组化技术在我们的独立样本中进行了验证。我们的结果表明，与人类正常乳腺组织相比，乳腺癌组织中TMPRSS3上调，TNFRSF11B下调。TMPRSS3和TNFRSF11B被证实与乳腺癌远处器官转移相关。此外，发现TMPRSS3上调伴随TNFRSF11B下调与较短的总生存期相关，提示预后不良。总之，TMPRSS3和TNFRSF11B可能具有潜在的预后价值，可作为乳腺癌患者的肿瘤生物标志物。
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