基于标记和无标记的表面增强拉曼散射用于病原体细菌检测：综述。

Label and label-free based surface-enhanced Raman scattering for pathogen bacteria detection: A review.

机构信息

Department of Preventative Medicine, Zhejiang Provincial Key Laboratory of Pathological and Physiological Technology, Medical School of Ningbo University, Ningbo, Zhejiang 315211, China.

Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou, Guangdong 510632, China.

出版信息

Biosens Bioelectron. 2017 Aug 15;94:131-140. doi: 10.1016/j.bios.2017.02.032. Epub 2017 Feb 28.

DOI:10.1016/j.bios.2017.02.032

PMID:28262610

Abstract

Rapid, accurate detection of pathogen bacteria is a highly topical research area for the sake of food safety and public health. Surface-enhanced Raman scattering (SERS) is being considered as a powerful and attractive technique for pathogen bacteria detection, due to its sensitivity, high speed, comparatively low cost, multiplexing ability and portability. This contribution aims to give a comprehensive overview of SERS as a technique for rapid detection of pathogen bacteria based on label and label-free strategies. A brief tutorial on SERS is given first of all. Then we summarize the recent trends and developments of label and label-free based SERS applied to detection of pathogen bacteria, including the relatively complete interpretation of SERS spectra. In addition, multifunctional SERS platforms for pathogen bacteria in matrix are discussed as well. Furthermore, an outlook of the work done and a perspective on the future directions of SERS as a reliable tool for real-time pathogen bacteria detection are given.

摘要

快速、准确地检测病原体细菌是食品安全和公共卫生领域的一个热门研究领域。表面增强拉曼散射（SERS）由于其灵敏度高、速度快、成本相对较低、可多重检测以及便携性等优点，被认为是一种强大而有吸引力的病原体细菌检测技术。本综述旨在全面概述基于标记和无标记策略的 SERS 技术在快速检测病原体细菌中的应用。首先，我们简要介绍了 SERS。然后，我们总结了基于标记和无标记的 SERS 在检测病原体细菌方面的最新趋势和进展，包括对 SERS 光谱的相对完整的解释。此外，我们还讨论了用于基质中病原体细菌的多功能 SERS 平台。此外，我们还对 SERS 作为一种可靠的实时病原体细菌检测工具的现有工作进行了展望，并对未来的发展方向进行了探讨。