Jafarzadeh Abdollah, Ahangar-Parvin Reyhane, Nemat Maryam, Taghipour Zahra, Shamsizadeh Ali, Ayoobi Fatemeh, Hassan Zuhair Mohammad
Department of Immunology, Medical School, Rafsanjan University of Medical Sciences, Rafsanjan, Iran; Department of Immunology, Medical School, Kerman University of Medical Sciences, Kerman, Iran; Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
Avicenna J Phytomed. 2017 Jan-Feb;7(1):54-65.
The main function of IL-12 is differentiation of naive T cells intoTh1 cells and TGF-β is a powerful immunoregulatory cytokine. The immunomodulatory and anti-inflammatory properties of ginger have also been reported in some studies. The aim of this study was to evaluate the effects of ginger extract on the expression of IL-12 and TGF-β in a model of experimental autoimmune encephalomyelitis (EAE).
EAE was induced in C57BL/6 mice by immunization with myelin oligodendroglial glycoprotein emulsified in complete Freund's adjuvant. The mice were administered intra-peritoneally with ginger extracts or PBS, from day +3 to +30. On day 31, mice were scarified and the expression of IL-12 and TGF-β mRNA in the spinal cord were determined by using real time-PCR. The serum levels of cytokines were measured by ELISA.
In PBS-treated EAE mice, the expression of IL-12 P35 and IL-12 P40 mRNA in the CNS and the mean serum levels of IL-12 were significantly higher than those of healthy group (p<0.001). In ginger-treated EAE mice, the expression of IL-12 mRNA and its serum levels were significantly lower as compared to PBS-treated EAE mice. No significant difference was observed between PBS-treated EAE mice and healthy group regarding the expression of TGF-β mRNA. In ginger (300 mg/kg)-treated EAE group, the expression of TGF-β mRNA and its serum levels were significantly higher in comparison to PBS-treated EAE mice (p<0.01 and p<0.05, respectively).
These results indicated that ginger extract modulates the expression of IL-12 and TGF-β in CNS and serum of EAE mice.
白细胞介素-12（IL-12）的主要功能是使初始T细胞分化为Th1细胞，而转化生长因子-β（TGF-β）是一种强大的免疫调节细胞因子。一些研究也报道了生姜的免疫调节和抗炎特性。本研究的目的是评估生姜提取物对实验性自身免疫性脑脊髓炎（EAE）模型中IL-12和TGF-β表达的影响。
用完全弗氏佐剂乳化的髓鞘少突胶质细胞糖蛋白免疫C57BL/6小鼠诱导EAE。从第3天至第30天，给小鼠腹腔注射生姜提取物或磷酸盐缓冲液（PBS）。在第31天，处死小鼠，通过实时定量聚合酶链反应（real time-PCR）测定脊髓中IL-12和TGF-β信使核糖核酸（mRNA）的表达。用酶联免疫吸附测定法（ELISA）检测细胞因子的血清水平。
在PBS处理的EAE小鼠中，中枢神经系统（CNS）中IL-12 P35和IL-12 P40 mRNA的表达以及IL-12的平均血清水平显著高于健康组（p<0.001）。与PBS处理的EAE小鼠相比，生姜处理的EAE小鼠中IL-12 mRNA的表达及其血清水平显著降低。在TGF-β mRNA的表达方面，PBS处理的EAE小鼠与健康组之间未观察到显著差异。与PBS处理的EAE小鼠相比，生姜（300毫克/千克）处理的EAE组中TGF-β mRNA的表达及其血清水平显著升高（分别为p<0.01和p<0.05）。
这些结果表明生姜提取物可调节EAE小鼠中枢神经系统和血清中IL-12和TGF-β的表达。
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