使用基质辅助激光解吸电离飞行时间质谱法对溶血曼氏杆菌主要基因型1和2进行快速分型。
Rapid typing of Mannheimia haemolytica major genotypes 1 and 2 using MALDI-TOF mass spectrometry.
作者信息
Loy John Dustin, Clawson Michael L
机构信息
University of Nebraska-Lincoln, Institute of Agriculture and Natural Resources, School of Veterinary Medicine and Biomedical Sciences, Lincoln, NE, USA.
United States Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research Center, Clay Center, NE, USA.
出版信息
J Microbiol Methods. 2017 May;136:30-33. doi: 10.1016/j.mimet.2017.03.002. Epub 2017 Mar 4.
Genotype 2M. haemolytica predominantly associate over genotype 1 with the lungs of cattle with respiratory disease and ICEs containing antimicrobial resistance genes. Distinct protein masses were detected by MALDI-TOF MS between genotype 1 and 2 strains. MALDI-TOF MS could rapidly differentiate genotype 2 strains in veterinary diagnostic laboratories.
2型溶血曼氏杆菌在患有呼吸道疾病的牛的肺部以及含有抗微生物耐药基因的整合性接合元件方面，比1型更占优势。通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）检测到1型和2型菌株之间存在不同的蛋白质质量。MALDI-TOF MS能够在兽医诊断实验室中快速区分2型菌株。
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