用于通过基质胶对三维球形癌细胞侵袭进行单细胞监测的共振波导光栅成像仪。

Resonant Waveguide Grating Imager for Single Cell Monitoring of the Invasion of 3D Speheroid Cancer Cells Through Matrigel.

作者信息

Febles Nicole K, Chandrasekaran Siddarth, Fang Ye

机构信息

Biochemical Technologies, Corning Research and Development Corporation, Corning Incorporated, Sullivan Park, Corning, NY, 14831, USA.

NanoScience Technology Center, Department of Mechanical, Materials and Aerospace Engineering, University of Central Florida, Orlando, FL, USA.

出版信息

Methods Mol Biol. 2017;1571:143-160. doi: 10.1007/978-1-4939-6848-0_10.

DOI:10.1007/978-1-4939-6848-0_10

PMID:28281255

Abstract

The invasion of cancer cells through their surrounding extracellular matrices is the first critical step to metastasis, a devastating event to cancer patients. However, in vitro cancer cell invasion is mostly studied using two-dimensional (2D) models. Three-dimensional (3D) multicellular spheroids may offer an advantageous cell model for cancer research and oncology drug discovery. This chapter describes a label-free, real-time, and single-cell approach to quantify the invasion of 3D spheroid colon cancer cells through Matrigel using a spatially resolved resonant waveguide grating imager.

摘要

癌细胞穿过其周围细胞外基质的侵袭是转移的首个关键步骤，而转移对癌症患者来说是一个灾难性事件。然而，体外癌细胞侵袭大多是使用二维（2D）模型进行研究的。三维（3D）多细胞球体可能为癌症研究和肿瘤学药物发现提供一种具有优势的细胞模型。本章介绍一种无标记、实时且针对单细胞的方法，该方法使用空间分辨共振波导光栅成像仪来量化3D球体结肠癌细胞通过基质胶的侵袭情况。