Takiguchi Keiko, Yasugi Sadao, Mizuno Takeo
The Zoological Institute, Faculty of Science, University of Tokyo, Hongo, 113, Tokyo, Japan.
Rouxs Arch Dev Biol. 1986 Oct;195(8):475-483. doi: 10.1007/BF00375887.
The avian stomach is composed of two distinct organs, the proventriculus and the gizzard. Pepsinogen expression in the proventricular and gizzard epithelia of chick embryos was investigated immunohistochemically with anti-embryonic chick pepsinogen (anti-ECPg) antiserum. In normal development, the ECPg antigen was expressed only in the glandular epithelial cells of the embryonic proventriculus from the 8th day of incubation onwards. However, both proventricular and gizzard epithelia of 6-day embryos expressed the ECPg antigen when recombined and cultured with the proventricular mesenchyme. Chronological studies revealed that the ECPg antigen was first detected in a few epithelial cells at 3 days of cultivation. The percentage of ECPg-positive cells among the total epithelial cells in each recombinant increased with the length of the culture period and all the glandular epithelial cells were positive at 9 days. During this process, the percentage of ECPg-positive cells in each cultured recombinant was similar in proventricular and gizzard epithelia. Moreover, both epithelia could express the ECPg antigen when recombined and cultured with the oesophageal or small-intestine mesenchyme for 9 days, though the percentage of ECPg-positive cells in each cultured recombinant was much lower than that in the cultured recombinant with the proventricular mesenchyme. These results indicate that the gizzard epithelium of 6-day chick embryos possesses a similar potential for pepsinogen expression as the proventricular epithelium of the same age.
鸟类的胃由两个不同的器官组成，即腺胃和肌胃。用抗胚胎鸡胃蛋白酶原（anti-ECPg）抗血清对鸡胚腺胃和肌胃上皮中的胃蛋白酶原表达进行了免疫组织化学研究。在正常发育过程中，从孵化第8天起，ECPg抗原仅在胚胎腺胃的腺上皮细胞中表达。然而，6日龄胚胎的腺胃和肌胃上皮在与腺胃间充质重组并培养时均表达ECPg抗原。时序研究表明，在培养3天时首次在少数上皮细胞中检测到ECPg抗原。每个重组体中ECPg阳性细胞在总上皮细胞中的百分比随培养时间的延长而增加，在9天时所有腺上皮细胞均呈阳性。在此过程中，每个培养重组体中腺胃和肌胃上皮中ECPg阳性细胞的百分比相似。此外，当与食管或小肠间充质重组并培养9天时，两种上皮均可表达ECPg抗原，尽管每个培养重组体中ECPg阳性细胞的百分比远低于与腺胃间充质培养的重组体。这些结果表明，6日龄鸡胚的肌胃上皮与同年龄的腺胃上皮具有相似的胃蛋白酶原表达潜能。
