Pope Phillip B, Vivekanand Vivekanand, Eijsink Vincent G H, Horn Svein J
Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Post Office Box 5003, 1432, Ås, Norway.
3 Biotech. 2013 Oct;3(5):407-414. doi: 10.1007/s13205-012-0097-x. Epub 2012 Oct 25.
Seaweed is a highly attractive marine crop for the production of biofuels, due to its rapid growth rate as well as high polysaccharide and low lignin content. One appealing exploitation route is the production of biogas by anaerobic digestion. Interestingly, despite the compositional differences between seaweed and lignocellulosic biomass, available data indicate that conditions and inocula traditionally used for the latter may work well for seaweed. To gain more insight into the underlying microbial processes, we have generated 16S rRNA gene amplicon pyrosequencing data to comparatively describe microbial communities in biogas digesters containing either the seaweed Saccharina latissima or wheat straw. The seaweed digesters gave better biogas yield and a higher relative abundance of core group Methanosaeta-affiliated Archaea. Conversely, variation in biomass had only minor abundance effects towards dominant bacterial lineages and influenced only low-abundant bacterial OTUs. Affiliations between dominant archaeal and bacterial phylotypes described here and previously identified anaerobic digestion core groups indicate that trends are beginning to emerge within these newly explored microbial ecosystems, the understanding of which is currently impeded by limited published datasets.
由于海藻生长速度快，多糖含量高且木质素含量低，因此它是一种极具吸引力的用于生产生物燃料的海洋作物。一种有吸引力的开发途径是通过厌氧消化生产沼气。有趣的是，尽管海藻和木质纤维素生物质在成分上存在差异，但现有数据表明，传统上用于后者的条件和接种物可能对海藻也适用。为了更深入了解潜在的微生物过程，我们生成了16S rRNA基因扩增子焦磷酸测序数据，以比较描述含有海藻海带或麦秸的沼气发酵罐中的微生物群落。海藻发酵罐产生了更好的沼气产量，并且与核心菌群甲烷八叠球菌属相关的古菌相对丰度更高。相反，生物质的变化对优势细菌谱系的丰度影响较小，仅影响低丰度细菌OTU。此处描述的优势古菌和细菌系统发育型与先前确定的厌氧消化核心菌群之间的联系表明，在这些新探索的微生物生态系统中开始出现一些趋势，目前有限的已发表数据集阻碍了对这些趋势的理解。
