[慢性牙周炎患者唾液蛋白酶谱分析]
[Analysis of salivary protease spectrum in chronic periodontitis].
作者信息
Qian Li, Xuedong Zhou, Yaping Fan, Tengyu Yang, Songtao Wu, Yu Yu, Jiao Chen, Ping Zhang, Yun Feng
机构信息
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
出版信息
Hua Xi Kou Qiang Yi Xue Za Zhi. 2017 Feb 1;35(1):37-42. doi: 10.7518/hxkq.2017.01.005.
OBJECTIVE
This study aimed to investigate the difference in salivary protease expression in patients with chronic periodontitis and normal individuals.
METHODS
The stimulating saliva in patients with chronic periodontitis and normal individuals were collected. Protein chip technology was adapted to analyze salivary protease spectrum.
RESULTS
Among the 34 proteases in the chip, disintegrin and metalloproteinase (ADAM)8, matrix metalloproteinase (MMP)-8, MMP-12, neprilysin/CD10, and uridylyl phosphate adenosine/urokinase showed a significantly increased concentration in the saliva of chronic periodontitis patients compared with those in the saliva of normal individuals (P<0.01). By contrast, the concentrations of ADAM9, a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)1, ADAMTS13, cathepsin B, E, L, V, X/Z/P, kallikrein 6, 7, 11, 13, MMP-9, proteinase 3, presenilin-1, and proprotein convertase 9 sharply decreased (P<0.05).
CONCLUSIONS
The results demonstrated that protease spectrum in the saliva of chronic periodontitis patients and normal individuals significantly differed. Analysis of salivary protease spectrum is a potential clinical method to examine, diagnose, and monitor chronic periodontitis.
目的
本研究旨在调查慢性牙周炎患者与正常个体唾液蛋白酶表达的差异。
方法
收集慢性牙周炎患者和正常个体的刺激性唾液。采用蛋白质芯片技术分析唾液蛋白酶谱。
结果
芯片中的34种蛋白酶中，与正常个体唾液相比，慢性牙周炎患者唾液中解整合素和金属蛋白酶（ADAM）8、基质金属蛋白酶（MMP）-8、MMP-12、中性肽链内切酶/CD10和尿苷磷酸腺苷/尿激酶浓度显著升高（P<0.01）。相比之下，含血小板反应蛋白基序的解整合素和金属蛋白酶（ADAMTS）1、ADAMTS13、组织蛋白酶B、E、L、V、X/Z/P、激肽释放酶6、7、11、13、MMP-9、蛋白酶3、早老素-1和前蛋白转化酶9的浓度急剧下降（P<0.05）。
结论
结果表明，慢性牙周炎患者与正常个体唾液中的蛋白酶谱存在显著差异。分析唾液蛋白酶谱是一种潜在用于检查、诊断和监测慢性牙周炎的临床方法。
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