DNA双链内的化学反应。溴化氰作为一种有效的寡脱氧核糖核苷酸偶联剂。
Chemical reactions within DNA duplexes. Cyanogen bromide as an effective oligodeoxyribonucleotide coupling agent.
作者信息
Sokolova N I, Ashirbekova D T, Dolinnaya N G, Shabarova Z A
机构信息
A.N. Belozersky Laboratory, Moscow State University, USSR.
出版信息
FEBS Lett. 1988 May 9;232(1):153-5. doi: 10.1016/0014-5793(88)80406-x.
PMID:2835262
Abstract
Cyanogen bromide was found to condense oligodeoxyribonucleotides on a complementary template in aqueous solution. Optimum conditions for this vigorous and effective reaction were developed. CNBr proved to be useful for incorporation of phosphoramidate or pyrophosphate internucleotide bonds in DNA duplexes.
摘要
发现溴化氰可使寡脱氧核糖核苷酸在水溶液中的互补模板上缩合。已确定了此剧烈且有效反应的最佳条件。事实证明，溴化氰对于在DNA双链体中引入氨基磷酸酯或焦磷酸核苷酸间键很有用。
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