Jeffries Matthew D, Gannon Travis W, Maxwell Patrick J
Department of Crop and Soil Sciences, North Carolina State University;
Department of Crop and Soil Sciences, North Carolina State University.
J Vis Exp. 2017 Mar 15(121):55182. doi: 10.3791/55182.
Plant canopies in established turfgrass systems can intercept an appreciable amount of sprayed pesticides, which can be transferred through various routes onto humans. For this reason, transferable pesticide residue experiments are required for registration and re-registration by the United States Environmental Protection Agency (USEPA). Although such experiments are required, limited specificity is required pertaining to experimental approach. Experimental approaches used to assess pesticide transfer to humans including hand wiping with cotton gloves, modified California roller (moving a roller of known mass over cotton cloth) and soccer ball roll (ball wrapped with sorbent strip) over three treated turfgrass species (creeping bentgrass, hybrid bermudagrass and tall fescue maintained at 0.4, 5 and 9 cm, respectively) are presented. The modified California roller is the most extensively utilized approach to date, and is best suited for use at low mowing heights due to its reproducibility and large sampling area. The soccer ball roll is a less aggressive transfer approach; however, it mimics a very common occurrence in the most popular international sport, and has many implications for nondietary pesticide exposure from hand-to-mouth contact. Additionally, this approach may be adjusted for other athletic activities with limited modification. Hand wiping is the best approach to transfer pesticides at higher mowing heights, as roller-based approaches can lay blades over; however, it is more subjective due to more variable sampling pressure. Utility of these methods across turfgrass species is presented, and additional considerations to conduct transferable pesticide residue research in turfgrass systems are discussed.
已建草坪草系统中的植物冠层能够截留相当数量的喷洒农药,这些农药可通过各种途径转移到人体上。因此,美国环境保护局(USEPA)要求进行可转移农药残留实验以用于登记和重新登记。尽管需要进行此类实验,但对实验方法的特异性要求有限。本文介绍了用于评估农药向人体转移的实验方法,包括用棉手套擦拭手部、改良加州滚筒法(将已知质量的滚筒在棉布上滚动)和足球滚动法(球上包裹吸附剂条),实验对象为三种经过处理的草坪草品种(匍匐翦股颖、杂交狗牙根和高羊茅,修剪高度分别保持在0.4厘米、5厘米和9厘米)。改良加州滚筒法是迄今为止使用最广泛的方法,由于其可重复性和较大的采样面积,最适合在低修剪高度下使用。足球滚动法是一种较为温和的转移方法;然而,它模拟了一项最受欢迎的国际运动中非常常见的情况,并且对通过手口接触造成的非饮食性农药暴露有许多影响。此外,只需进行有限的修改,这种方法就可以适用于其他体育活动。在较高修剪高度下,擦拭手部是转移农药的最佳方法,因为基于滚筒的方法可能会使草叶倒伏;然而,由于采样压力变化较大,这种方法主观性更强。本文展示了这些方法在不同草坪草品种中的实用性,并讨论了在草坪草系统中进行可转移农药残留研究的其他注意事项。