异源5'侧翼区域不支持黑腹果蝇tRNA(Val3b)基因的体外模板活性。

Heterologous 5' flanking regions do not support in vitro template activity of a Drosophila melanogaster tRNA(Val3b) gene.

作者信息

Mackay R M, Horvath D, Duncan L, Spiegelman G B

机构信息

Department of Microbiology, University of British Columbia, Vancouver, Canada.

出版信息

Nucleic Acids Res. 1988 May 11;16(9):4137-53. doi: 10.1093/nar/16.9.4137.

DOI:10.1093/nar/16.9.4137

PMID:2836812

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC336580/

Abstract

The 5' and 3' structure of a Drosophila tRNA(Val3b) gene was investigated to examine the defect which caused the extremely low in vitro transcription template activity of the gene. Recombinant genes were constructed linking 5' and 3' flanking regions from tRNA genes which were active in vitro templates (tRNA(Val4), tRNA(Arg), tRNA(Ser7)) to the tRNA(Val3b) gene. None of the recombinant genes were effective in vitro templates. The defect in tRNA(Val3b) was demonstrated to reside in the 5' flanking region of the gene and deletion analysis indicated that no specific transcription inhibitor sequence was present 5' to the gene. The data suggest that the effect of 5' flanking sequences on in vitro transcription of the tRNA(Val3b) gene requires a specific relationship between the tRNA gene and the flanking sequence.

摘要

对果蝇tRNA（Val3b）基因的5'和3'结构进行了研究，以检查导致该基因体外转录模板活性极低的缺陷。构建了重组基因，将来自在体外模板中具有活性的tRNA基因（tRNA（Val4）、tRNA（Arg）、tRNA（Ser7））的5'和3'侧翼区域连接到tRNA（Val3b）基因上。这些重组基因均不是有效的体外模板。结果表明，tRNA（Val3b）的缺陷位于该基因的5'侧翼区域，缺失分析表明该基因5'端不存在特异性转录抑制序列。数据表明，5'侧翼序列对tRNA（Val3b）基因体外转录的影响需要tRNA基因与侧翼序列之间存在特定关系。