[OCT4、Notch1和DLL4在上皮性卵巢癌中的表达及其临床意义]
[Expressions of OCT4, Notch1 and DLL4 and their clinical implications in epithelial ovarian cancer].
作者信息
Yu Lan, Jiao Yun-Jie, Zhou Lei, Song Wen-Qing, Wu Shi-Wu, Wang Dan-Na
机构信息
Department of Pathology, the first Affiliated Hospital of Bengbu Medical College, Bengbu Medical College, Anhui Key Laboratory of Infection and Immunology, Bengbu Medical College, Bengbu 233000, China. E-mail:
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2016 Apr 20;37(4):444-450. doi: 10.3969/j.issn.1673-4254.2017.04.04.
OBJECTIVE
To investigate the correlations among OCT4, Notch1 and DLL4 and their association with the clinicopathological features of patients with epithelial ovarian cancer (EOC).
METHODS
A total of 207 specimens of EOC and 65 specimens of benign ovarian epithelial tumor tissues were examined for expressions of OCT4, Notch1 and DLL4 proteins using immunohistochemistry.
RESULTS
The positivity rates of OCT4, Notch1 and DLL4 in EOC tissues were 60.0%, 61.8% and 60.9%, respectively, significantly higher than the rates in benign epithelial tumor tissues (9.2%, 6.2%, and 0, respectively; P<0.05). The expressions of OCT4, Notch1 and DLL4 in EOC were significantly correlated with tumor differentiation, FIGO stage, and lymph node metastasis (P<0.05). DLL4 was positively correlated with OCT4 and Notch1 expressions (r=0.758 and 0.704, respectively, P<0.001), and the latter two were also positively correlated (r=0.645, P<0.001). Overexpressions of OCT4, Notch1 and DLL4 were associated with a poor prognosis, and the survival rate was significantly lower in patients positive for OCT4, Notch1, and DLL4 than in the negative patients (P<0.05). FIGO stage and expressions of OCT4 and DLL4 were independent prognostic factors of EOC (P<0.05).
CONCLUSION
The expressions of OCT4, Notch1 and DLL4 are correlated with the differentiation, lymph node metastasis, clinical stage and prognosis of EOC. Combined detection of the 3 proteins has an important value in predicting the progression and prognosis of EOC.
目的
探讨八聚体结合转录因子4（OCT4）、Notch1和Delta样蛋白4（DLL4）之间的相关性及其与上皮性卵巢癌（EOC）患者临床病理特征的关系。
方法
采用免疫组织化学法检测207例EOC标本和65例良性卵巢上皮性肿瘤组织中OCT4、Notch1和DLL4蛋白的表达。
结果
EOC组织中OCT4、Notch1和DLL4的阳性率分别为60.0%、61.8%和60.9%，显著高于良性上皮性肿瘤组织（分别为9.2%、6.2%和0；P<0.05）。EOC中OCT4、Notch1和DLL4的表达与肿瘤分化、国际妇产科联盟（FIGO）分期及淋巴结转移显著相关（P<0.05）。DLL4与OCT4和Notch1表达呈正相关（r分别为0.758和0.704，P<0.001），后两者也呈正相关（r=0.645，P<0.001）。OCT4、Notch1和DLL4的过表达与预后不良相关，OCT4、Notch1和DLL4阳性患者的生存率显著低于阴性患者（P<0.05）。FIGO分期以及OCT4和DLL4的表达是EOC的独立预后因素（P<0.05）。
结论
OCT4、Notch1和DLL4的表达与EOC的分化、淋巴结转移、临床分期及预后相关。联合检测这3种蛋白对预测EOC的进展和预后具有重要价值。
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