College of Fisheries, Henan Normal University, Xinxiang, 453007, People's Republic of China.
Synthetic Biology Remaking Engineering & Application Laboratory (SynBreal), College of Life Sciences and Technology, Xinxiang Medical University, Xinxiang, 453003, People's Republic of China.
Antonie Van Leeuwenhoek. 2017 Sep;110(9):1149-1155. doi: 10.1007/s10482-017-0887-6. Epub 2017 May 12.
The taxonomic status was determined of two actinomycetes, designated CFH S0057 and CFH S0065, that were isolated from soil samples collected from an extinct volcano in Tengchong county, Yunnan province, south-west China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains CFH S0057 and CFH S0065 belong to the genus Nocardia and formed a single clade within this genus. The two isolates were able to grow at 4-45 °C, pH 5.0-7.0 and with a NaCl tolerance up to 5.0% (w/v). The whole-cell hydrolysates were rich in meso-diaminopimelic acid, galactose, arabinose and fructose. Mycolic acids were present. Strains CFH S0057 and CFH S0065 exhibited a menaquinone system with MK-8 (H, ω cyclo), and the major polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and one unidentified phospholipid. Major fatty acids were C, Summed features 3, C ω9c and C 10-methyl (TBSA). The genomic DNA G + C contents of strains CFH S0057 and CFH S0065 were 65.7 and 66.1 mol%, respectively. The combined genotypic, phenotypic and chemotaxonomic results indicated the isolates are considered to represent a novel species of the genus Nocardia, for which the name Nocardia tengchongensis sp. nov. is proposed. The type strain is CFH S0057 (= KCTC 29485 = JCM 30083).
两株放线菌 CFH S0057 和 CFH S0065 是从中国西南部云南省腾冲县一座死火山采集的土壤样本中分离出来的，其分类地位已确定。基于 16S rRNA 基因序列的系统发育分析表明，菌株 CFH S0057 和 CFH S0065 属于诺卡氏菌属，在该属内形成一个单独的分支。两株分离株能够在 4-45°C、pH5.0-7.0 和耐受高达 5.0%（w/v）的 NaCl 条件下生长。全细胞水解物富含 meso-二氨基庚二酸、半乳糖、阿拉伯糖和果糖。存在分枝菌酸。菌株 CFH S0057 和 CFH S0065 表现出一个menaquinone 系统，MK-8（H，ω 环），主要极性脂类由双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰肌醇、磷脂酰肌醇甘露糖苷和一种未鉴定的磷脂组成。主要脂肪酸为 C、Summed features 3、C ω9c 和 C 10-methyl (TBSA)。菌株 CFH S0057 和 CFH S0065 的基因组 DNA G+C 含量分别为 65.7 和 66.1 mol%。综合表型、基因型和化学分类学结果表明，这些分离株被认为代表了诺卡氏菌属的一个新种，现提议将其命名为腾冲诺卡氏菌（Nocardia tengchongensis sp. nov.）。模式菌株为 CFH S0057（=KCTC 29485=JCM 30083）。
