Mitsui Toshihito, Ito Tomoyuki, Sasaki Yusuke, Kawasaki Takashi, Nakamura Takeshi, Nishimura Yukihide, Ibusuki Tatsuru, Higuchi Yukiharu, Hosoe Sayoko, Ito Fumiaki, Tajima Fumihiro
Department of Rehabilitation Medicine and Sports Medical Center, Wakayama Medical University School of Medicine, Wakayama City, Japan.
Faculty of Sports Science, Nihon Fukushi University, Mihama, Aichi, Japan.
Spinal Cord Ser Cases. 2017 May 11;3:17015. doi: 10.1038/scsandc.2017.15. eCollection 2017.
We reported previously that exercise significantly increases plasma adrenaline and oxidized low-density lipoprotein (oxLDL) in healthy subjects but not in persons with spinal cord injury (SCI). Since oxLDL and adrenaline levels are associated with oxidant/antioxidant balance, and exercise training elicits production of reactive oxygen species, we elucidated the effects of exercise on adrenaline, oxidant/antioxidant balance and oxLDL in individuals with SCI.
Eight subjects with cervical spinal cord injury (CSCI) and nine subjects with lower lesion of SCI (lower SCI (LSCI)) participated in a wheelchair half marathon race, and blood samples were collected before (pre), immediately after (post) and 1 h after the race (post 1 h). The blood samples were used to determine adrenaline, derivatives of reactive oxygen metabolites (d-ROMs) and biological antioxidant potential (BAP), both as markers for oxidant/antioxidant balance.
Pre-serum oxLDL levels were 147.2±8.1 and 97.0±10.4 U l (mean±s.e.m.) in LCSI and CSCI subjects, respectively, and remained stable throughout the study. Adrenaline levels were higher in LSCI athletes than in CSCI athletes, especially post half marathon. Serum d-ROMs level did not change between pre and post in both groups. The mean BAP was significantly higher in LSCI than in CSCI subjects (2574±94.6 vs 2118±94.6 μmol l) at post, whereas the oxidative stress index (d-ROMs/BAP) was similar in the two groups throughout the study. In conclusion, exercise did not increase the d-ROMs or d-ROMs/BAP ratio in CSCI and LSCI subjects. The lack of increase in the plasma oxLDL level in SCI subjects was not due to the lack of response of adrenaline to exercise.
我们之前报道过，运动可显著提高健康受试者的血浆肾上腺素和氧化型低密度脂蛋白（oxLDL）水平，但脊髓损伤（SCI）患者却无此变化。由于oxLDL和肾上腺素水平与氧化/抗氧化平衡相关，且运动训练会引发活性氧的产生，因此我们阐明了运动对SCI患者肾上腺素、氧化/抗氧化平衡及oxLDL的影响。
8名颈脊髓损伤（CSCI）患者和9名下位脊髓损伤（LSCI）患者参加了一场轮椅半程马拉松比赛，并在比赛前（pre）、比赛结束后即刻（post）及比赛结束后1小时（post 1 h）采集血样。血样用于测定肾上腺素、活性氧代谢物衍生物（d-ROMs）和生物抗氧化能力（BAP），二者均为氧化/抗氧化平衡的标志物。
LSCI和CSCI患者的血清oxLDL水平在研究开始前分别为147.2±8.1和97.0±10.4 U l（平均值±标准误），且在整个研究过程中保持稳定。LSCI运动员的肾上腺素水平高于CSCI运动员，尤其是在半程马拉松赛后。两组患者血清d-ROMs水平在赛前和赛后均无变化。赛后LSCI患者的平均BAP显著高于CSCI患者（2574±94.6对2118±94.6 μmol l），而整个研究过程中两组的氧化应激指数（d-ROMs/BAP）相似。总之，运动并未增加CSCI和LSCI患者的d-ROMs或d-ROMs/BAP比值。SCI患者血浆oxLDL水平未升高并非由于肾上腺素对运动缺乏反应。
