由DNA编程的细胞大小的机械敏感和生物传感隔室。
Cell-sized mechanosensitive and biosensing compartment programmed with DNA.
作者信息
Majumder Sagardip, Garamella Jonathan, Wang Ying-Lin, DeNies Maxwell, Noireaux Vincent, Liu Allen P
机构信息
Department of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan, USA.
出版信息
Chem Commun (Camb). 2017 Jun 29;53(53):7349-7352. doi: 10.1039/c7cc03455e.
Abstract
The bottom-up construction of cell-sized compartments programmed with DNA that are capable of sensing the chemical and physical environment remains challenging in synthetic cell engineering. Here, we construct mechanosensitive liposomes with biosensing capability by expressing the E. coli channel MscL and a calcium biosensor using cell-free expression.
摘要
在合成细胞工程中，利用DNA对细胞大小的隔室进行自下而上的构建，使其能够感知化学和物理环境，仍然具有挑战性。在这里，我们通过无细胞表达来表达大肠杆菌通道MscL和钙生物传感器，构建具有生物传感能力的机械敏感脂质体。
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1
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2
Portable, On-Demand Biomolecular Manufacturing.
Cell. 2016 Sep 22;167(1):248-259.e12. doi: 10.1016/j.cell.2016.09.013.
3
Mechanically activated artificial cell by using microfluidics.
Sci Rep. 2016 Sep 9;6:32912. doi: 10.1038/srep32912.
4
Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with in vitro synthesized and assembled ribosomes.
Chem Commun (Camb). 2016 Apr 7;52(31):5467-9. doi: 10.1039/c6cc00223d.
5
Expanding the amino acid repertoire of ribosomal polypeptide synthesis via the artificial division of codon boxes.
Nat Chem. 2016 Apr;8(4):317-25. doi: 10.1038/nchem.2446. Epub 2016 Feb 1.
6
The All E. coli TX-TL Toolbox 2.0: A Platform for Cell-Free Synthetic Biology.
ACS Synth Biol. 2016 Apr 15;5(4):344-55. doi: 10.1021/acssynbio.5b00296. Epub 2016 Feb 9.
7
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Nat Commun. 2016 Jan 20;7:10366. doi: 10.1038/ncomms10366.
8
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Methods. 2015 Sep 15;86:60-72. doi: 10.1016/j.ymeth.2015.05.020. Epub 2015 May 27.
9
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Methods Cell Biol. 2015;128:303-18. doi: 10.1016/bs.mcb.2015.01.014. Epub 2015 Apr 8.
10
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Biotechnol Bioeng. 2015 Aug;112(8):1663-72. doi: 10.1002/bit.25587. Epub 2015 Jun 16.
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