Bisetto Shayne P, Carregaro Adriano B, Nicolai André E S, Bressan Thais F, Leal William P, Xavier Nathalia V, Leal Diego F, Andrade André F C
Department of Veterinary Science, School of Animal Science and Food Engineering, University of São Paulo, Pirassununga, SP, Brazil.
Department of Veterinary Science, School of Animal Science and Food Engineering, University of São Paulo, Pirassununga, SP, Brazil.
Vet Anaesth Analg. 2017 May;44(3):594-599. doi: 10.1016/j.vaa.2016.09.003. Epub 2017 Jan 11.
To evaluate the effect of hyaluronidase on uptake, duration and speed of elimination of xylazine-tiletamine-zolazepam administered in the subcutaneous fat over the dorsal lumbar region of swine.
Blinded, randomized, crossover study.
Six healthy Landrace/Large White pigs weighing 132±24 kg (mean±standard deviation).
Animals were administered xylazine (1 mg kg) and tiletamine-zolazepam (8 mg kg) (control treatment, CON), or xylazine-tiletamine-zolazepam at the same doses with hyaluronidase (400 IU) (treatment HYA). The treatments were administered into the dorsal lumbar adipose tissue, 2.5-3.0 cm laterally from the spinous process of the second lumbar vertebra. The latency, anesthesia and recovery periods were measured. Heart rate, noninvasive systolic, diastolic, and mean arterial pressures, respiratory rate, hemoglobin oxygen saturation and rectal temperature were recorded every 10 minutes for up to 50 minutes.
One animal in CON and one animal in HYA were responsive to stimulation and did not allow safe handling. No significant difference was found between treatments for latency (CON 11.3±5.9 minutes, HYA 7.4±5.1 minutes) and anesthesia (CON 53±53 minutes, HYA 49±38 minutes) periods. Recovery period was shorter in HYA (9±6 minutes) than in CON (32±16 minutes) (p < 0.05). Physiological variables were not significantly changed over time and were within accepted normal clinical limits for the species in both treatments.
Hyaluronidase (400 IU) administered into adipose tissue in pigs did not reduce the latency and duration of dissociative anesthesia, but was associated with faster recovery.
评估透明质酸酶对在猪背腰部皮下脂肪中注射赛拉嗪-替来他明-唑拉西泮后的摄取、持续时间及消除速度的影响。
盲法、随机、交叉研究。
6头健康的长白猪/大白猪，体重132±24千克（均值±标准差）。
给动物注射赛拉嗪（1毫克/千克）和替来他明-唑拉西泮（8毫克/千克）（对照处理，CON），或相同剂量的赛拉嗪-替来他明-唑拉西泮与透明质酸酶（400国际单位）（处理HYA）。将处理药物注射到第二腰椎棘突外侧2.5 - 3.0厘米处的背腰部脂肪组织中。测量潜伏期、麻醉期和恢复期。每10分钟记录心率、无创收缩压、舒张压和平均动脉压、呼吸频率、血红蛋白氧饱和度及直肠温度，持续记录50分钟。
CON组和HYA组各有1只动物对刺激有反应，无法安全处理。处理组之间在潜伏期（CON组11.3±5.9分钟，HYA组7.4±5.1分钟）和麻醉期（CON组53±53分钟，HYA组49±38分钟）未发现显著差异。HYA组的恢复期（9±6分钟）比CON组（32±16分钟）短（p<0.05）。两种处理中，生理变量随时间无显著变化，且均在该物种公认的正常临床范围内。
在猪脂肪组织中注射透明质酸酶（400国际单位）不会缩短分离麻醉的潜伏期和持续时间，但可加快恢复速度。
