类风湿关节炎患者外周血CCR6⁺CD4⁺和CD4⁺CD25⁺T细胞中芳烃受体表达失衡

Unbalanced expression of aryl hydrocarbon receptor in peripheral blood CCR6CD4 and CD4CD25T cells of rheumatoid arthritis.

作者信息

Cheng Lin, Qian Long, Tan Yue, Wang Guo-Sheng, Li Xiao-Mei, Li Xiang-Pei, Luo Chao-Yin

机构信息

Anhui Medical University, The Second Affiliated Hospital, Department of Rheumatology and Immunology, Hefei, China.

出版信息

Rev Bras Reumatol Engl Ed. 2017 May-Jun;57(3):190-196. doi: 10.1016/j.rbre.2016.07.002. Epub 2016 Jul 22.

DOI:10.1016/j.rbre.2016.07.002

PMID:28535889

Abstract

OBJECTIVE

The goal of this study was to analyze the role of aryl hydrocarbon receptor in peripheral blood CCR6CD4 and CD4CD25T cells of patients with rheumatoid arthritis.

METHODS

Flow cytometry was applied to determine the proportion of AhR positive cells in CCR6CD4T, CD4CD25T and peripheral blood peripheral mononuclear cells from each subject. AhR mRNA and CYP1A1 mRNA relative expression levels were tested by real-time PCR.

RESULTS

The percentage of AhR positive cells in peripheral blood mononuclear cells was higher in RA group than that in healthy cases [(35.23±10.71)% vs. (18.83±7.32)%, p<0.01]. The expression levels of AhR and CYP1A1 were both increased in patients with RA while compared to controls [(3.71±1.63) vs. (2.00±1.27), p=0.002; (2.62±2.08) vs. (0.62±0.29), p<0.01, respectively]. In RA patients, the percentage of AhR positive cells in CD4CD25T cells was significantly lower than that from controls [17.90 (6.10±80.10)% vs. (52.49±19.18)%, p<0.01]; In healthy controls, the percentage of AhR positive cells in CD4CD25T cells was significantly higher than that in CCR6CD4T cells, and was also significantly higher than that in PBMCs [(52.49±19.18)% vs. (23.18±5.62)% vs. (18.06±7.80)%, X=24.03, p<0.01]; in RA patients, the percentage of AhR positive cells in CCR6CD4T cells was significantly increased than that in CD4CD25T cells and PBMCs [(46.02±14.68)% vs. 17.90 (6.10±80.10)% vs. (34.22±10.33)%, X=38.29, p<0.01]; Nevertheless, no statistically significant relationship was found between clinical data and AhR positive cells in CCR6CD4T and CD4CD25T cells.

CONCLUSION

AhR may participate in the pathological progress of RA by controlling the differentiation of Th17 and Treg cells in peripheral blood.

摘要

目的

本研究旨在分析芳烃受体在类风湿关节炎患者外周血CCR6⁺CD4⁺和CD4⁺CD25⁺T细胞中的作用。

方法

应用流式细胞术检测各研究对象CCR6⁺CD4⁺T、CD4⁺CD25⁺T细胞及外周血单个核细胞中AhR阳性细胞的比例。采用实时荧光定量PCR检测AhR mRNA和CYP1A1 mRNA的相对表达水平。

结果

类风湿关节炎组外周血单个核细胞中AhR阳性细胞百分比高于健康对照组[（35.23±10.71）% 对（18.83±7.32）%，p<0.01]。与对照组相比，类风湿关节炎患者AhR和CYP1A1的表达水平均升高[分别为（3.71±1.63）对（2.00±1.27），p=0.002；（2.62±2.08）对（0.62±0.29），p<0.01]。类风湿关节炎患者CD4⁺CD25⁺T细胞中AhR阳性细胞百分比显著低于对照组[17.90（6.10±80.10）% 对（52.49±19.18）%，p<0.01]；在健康对照组中，CD4⁺CD25⁺T细胞中AhR阳性细胞百分比显著高于CCR6⁺CD4⁺T细胞，也显著高于外周血单个核细胞[（52.49±19.18）% 对（23.18±5.62）% 对（18.06±7.80）%，X=24.03，p<0.01]；在类风湿关节炎患者中，CCR6⁺CD4⁺T细胞中AhR阳性细胞百分比显著高于CD4⁺CD25⁺T细胞和外周血单个核细胞[（46.02±14.68）% 对 17.90（6.10±80.10）% 对（34.22±10.33）%，X=38.29，p<0.01]；然而，临床数据与CCR6⁺CD4⁺T和CD4⁺CD25⁺T细胞中AhR阳性细胞之间未发现统计学上的显著关系。