通过液相色谱-四极杆飞行时间串联质谱（LC-QTOF MS/MS）分析研究波斯青柠（Citrus latifolia）果实生长过程中极性组分的成分变化。
Changes in the composition of the polar fraction of Persian lime (Citrus latifolia) during fruit growth by LC-QTOF MS/MS analysis.
作者信息
Ledesma-Escobar C A, Priego-Capote F, Robles-Olvera V J, Luque de Castro M D
机构信息
Department of Analytical Chemistry, Annex C-3, Campus of Rabanales, University of Córdoba, E-14071 Córdoba, Spain; University of Córdoba Agroalimentary Excellence Campus, ceiA3, Campus of Rabanales, E-14071 Córdoba, Spain; Chemical and Biochemical Engineering Department, Technological Institute of Veracruz, Av. Miguel Ángel de Quevedo 2779, Veracruz, Ver. 91897, Mexico.
Department of Analytical Chemistry, Annex C-3, Campus of Rabanales, University of Córdoba, E-14071 Córdoba, Spain; University of Córdoba Agroalimentary Excellence Campus, ceiA3, Campus of Rabanales, E-14071 Córdoba, Spain; Maimónides Institute of Biomedical Research (IMIBIC), Reina Sofía Hospital, University of Córdoba, E-14014 Córdoba, Spain.
出版信息
Food Chem. 2017 Nov 1;234:262-268. doi: 10.1016/j.foodchem.2017.05.009. Epub 2017 May 4.
Citrus possess a large number of bioactive compounds mainly studied in ripe fruits. Few studies have focused on evolution of metabolites during fruit growth. In this study, fruits were sampled from weeks 1-14 of the ripening process. Polar extracts were obtained from all collected samples and analysed by liquid chromatography-tandem mass spectrometry. Analysis of variance applied to the dataset indicated that the relative concentration of 394 out of 423 molecular entities changed significantly during maturation. Principal component analysis showed a clear separation among samples from different weeks and revealed the main compounds responsible for differentiation. Additionally, 72 metabolites were tentatively identified and changes in their relative concentration during growth were individually analysed. The observed trends in relative concentrations of representative metabolites during the growth process are discussed.
柑橘含有大量主要在成熟果实中研究的生物活性化合物。很少有研究关注果实生长过程中代谢物的演变。在本研究中，在成熟过程的第1至14周采集果实样本。从所有采集的样本中获得极性提取物，并通过液相色谱 - 串联质谱法进行分析。对数据集进行的方差分析表明，423个分子实体中的394个的相对浓度在成熟过程中发生了显著变化。主成分分析显示不同周的样本之间有明显的分离，并揭示了导致差异的主要化合物。此外，初步鉴定了72种代谢物，并单独分析了它们在生长过程中相对浓度的变化。讨论了生长过程中代表性代谢物相对浓度的观察趋势。
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