Institute of Animal Nutrition, Feed Engineering Research Centre of Sichuan Province, Sichuan Agricultural University, Huimin Road 211#, Wenjiang District, Chengdu, Sichuan, 611130, China.
Chinese Chelota Group, Liangshui Industrial Estate, Jinyu District, Guanghan, Sihuan, 618300, China.
Biol Trace Elem Res. 2018 Feb;181(2):347-360. doi: 10.1007/s12011-017-1060-9. Epub 2017 May 30.
Manganese (Mn) is an essential nutrient for both host and pathogen. Recent studies have demonstrated the nutritional immunity of Mn against Salmonella infection in mammals. To investigate the effect of high dietary Mn on immune responses of broilers following Salmonella challenge, 144 1-day-old male broilers were fed a basal diet (containing 20.04 mg Mn/kg) plus an additional 40 (the control group) or 400 mg Mn/kg (the H-Mn group) for 7 days. The 72 broilers in each group were then orally inoculated with 5 × 10 CFUs of Salmonella typhimurium (ATCC#14028) or phosphate-buffered saline. Peripheral blood, spleens, cecal tonsils, and bursa of Fabricius were collected from Salmonella-inoculated and Salmonella-noninoculated broilers (n = 6) at 2 days post inoculation (2 DPI) and 7 days post inoculation (7 DPI). Peripheral blood lymphocyte subpopulations were determined by flow cytometry. The messenger RNA (mRNA) abundance of genes was determined by quantitative real-time polymerase chain reaction. Salmonella counts were higher (P < 0.05) in the H-Mn group than that in the control group at 2 DPI in the cecal contents of Salmonella-inoculated broilers. High dietary Mn increased CD3CD4 and CD3CD8 percentages in the peripheral blood of Salmonella-inoculated broilers at 2 DPI. Salmonella inoculation increased interleukin (IL)-6 mRNA expression in spleens and bursa of Fabricius at 2 DPI and increased IL-1β and IL-6 mRNA expression in cecal tonsils at 7 DPI in the H-Mn group. These changes were not observed in the control group. High dietary Mn increased interferon-γ (IFN-γ) in spleens and decreased IFN-γ and IL-12 mRNA expression in cecal tonsils of Salmonella-inoculated broilers at 2 DPI. High dietary Mn decreased IL-17 mRNA expression in the bursa of Fabricius at 7 DPI, but increased this expression in cecal tonsils at 2 and 7 DPI in Salmonella-inoculated broilers. These results suggested that dietary Mn level affected T helper (Th) 1-cytokine reaction in spleens and cecal tonsils, and Th17-mediated immunity in cecal tonsils and the bursa of Fabricius of broilers when challenged with Salmonella.
锰 (Mn) 是宿主和病原体都必需的营养物质。最近的研究表明，Mn 具有抗沙门氏菌感染的营养免疫作用。为了研究高膳食 Mn 对沙门氏菌攻毒后肉鸡免疫反应的影响，将 144 只 1 日龄雄性肉鸡分别用基础日粮（含 20.04 mg Mn/kg）+40 mg Mn/kg（对照组）或 400 mg Mn/kg（高 Mn 组）饲养 7 天。然后，每组 72 只鸡分别用 5×10 CFUs 鼠伤寒沙门氏菌（ATCC#14028）或磷酸盐缓冲液经口接种。在攻毒后 2 天（2 DPI）和 7 天（7 DPI），从沙门氏菌攻毒和未攻毒的肉鸡（每组 6 只）中采集外周血、脾脏、盲肠扁桃体和法氏囊。采用流式细胞术检测外周血淋巴细胞亚群。采用实时定量聚合酶链反应（qRT-PCR）测定基因的信使 RNA（mRNA）丰度。在沙门氏菌攻毒的肉鸡盲肠内容物中，高 Mn 组的 2 DPI 时的沙门氏菌计数高于对照组（P<0.05）。高 Mn 组在 2 DPI 时，外周血中的 CD3CD4 和 CD3CD8 百分比增加。沙门氏菌攻毒增加了脾脏和法氏囊中白细胞介素（IL）-6 mRNA 的表达（2 DPI），增加了盲肠扁桃体中 IL-1β 和 IL-6 mRNA 的表达（7 DPI）。在对照组中未观察到这些变化。高 Mn 组增加了脾脏中的干扰素-γ（IFN-γ），降低了沙门氏菌攻毒肉鸡盲肠扁桃体中 IFN-γ 和 IL-12 mRNA 的表达（2 DPI）。高 Mn 组减少了 7 DPI 时法氏囊中 IL-17 mRNA 的表达，但增加了沙门氏菌攻毒肉鸡盲肠扁桃体中 2 和 7 DPI 时的 IL-17 mRNA 的表达。这些结果表明，膳食 Mn 水平影响了沙门氏菌攻毒后肉鸡脾脏和盲肠扁桃体中的 Th1-细胞因子反应，以及 Th17 介导的盲肠扁桃体和法氏囊的免疫反应。
Zotero 插件