Youssefi M R, Abouhosseini Tabari M, Moghadamnia A A
Department of Veterinary Parasitology, Babol Branch, Islamic Azad University, Babol, Iran.
Department of Veterinary Pharmacology, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran.
Iran J Vet Res. 2017 Winter;18(1):25-29.
In Iranian folk medicine has been used for treatment of parasite infections in human and animals. The present study was designed to evaluate the and effects of essential oil (EO) against . were recovered by wet mount method from infected native pigeons. The assays were accomplished in multi-well plates containing metronidazole (MTZ) as a standard antitrichomonal and EO in final concentrations of 2.5, 5, 10, 20, 50, and 100 μg/ml of culture medium containing 10 parasites. The assay was performed on 40 experimentally infected pigeons receiving 25 and 50 mg/kg of MTZ and EO for 7 successive days. Gas chromatographic (GC) analysis was performed to reveal chemical constituents of the EO. At 20 µg/ml, MTZ resulted in no viable trophozoite in culture medium after 24 h incubation period. While the 24 h MIC of EO was 10 µg/ml. Treatment with EO at dose of 50 mg/kg after 4 days led to full recovery of infected pigeons but for MTZ at the same dose 5 days were spent. Major constituents of EO were α-thujone (31.5%) and β-thujone (11.92%). Data of the present study introduced as a natural potent antitrichomonal agent effective against .
在伊朗民间医学中，它已被用于治疗人和动物的寄生虫感染。本研究旨在评估[植物名称]精油（EO）对[寄生虫名称]的[具体作用1]和[具体作用2]效果。通过湿涂片法从感染的本地鸽子中分离出[寄生虫名称]。在含有甲硝唑（MTZ）作为标准抗滴虫药物和最终浓度为2.5、5、10、20、50和100μg/ml的EO的多孔板中进行[具体实验1]测定，培养基中含有10个寄生虫。对40只实验感染的鸽子进行[具体实验2]测定，连续7天给予25和50mg/kg的MTZ和EO。进行气相色谱（GC）分析以揭示EO的化学成分。在20μg/ml时，MTZ在孵育24小时后导致培养基中没有存活的滋养体。而EO的24小时最低抑菌浓度为10μg/ml。以50mg/kg的剂量用EO治疗4天后，感染的鸽子完全康复，但相同剂量的MTZ则需要5天。EO的主要成分是α-侧柏酮（31.5%）和β-侧柏酮（11.92%）。本研究的数据表明[植物名称]是一种对[寄生虫名称]有效的天然强效抗滴虫剂。
