尿路感染的快速抗生素敏感性检测
Rapid Antibiotic Susceptibility Testing for Urinary Tract Infections.
作者信息
Mezger Anja, Nilsson Mats, Andersson Dan I
机构信息
Broad Institute of MIT and Harvard, Cambridge, MA, USA.
Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
出版信息
Methods Mol Biol. 2017;1616:147-153. doi: 10.1007/978-1-4939-7037-7_9.
Antibiotic susceptibility testing is important to guide clinicians in their choice of antibiotic used for treatment of bacterial infections. Current methods are time-consuming and more rapid alternatives are needed. Here, we describe a novel rapid method for antibiotic susceptibility testing which combines phenotypic and genotypic measurements. The use of padlock probes and rolling circle amplification allows for fast and precise determination of antibiotic susceptibilities as well as species identification.
抗生素敏感性测试对于指导临床医生选择用于治疗细菌感染的抗生素非常重要。目前的方法耗时较长，因此需要更快速的替代方法。在此，我们描述了一种结合表型和基因型测量的新型抗生素敏感性快速测试方法。使用锁式探针和滚环扩增能够快速、精确地确定抗生素敏感性以及菌种鉴定。
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