中国江苏省HIV阳性同性恋男性中人类疱疹病毒8型感染的高血清阳性率

High Seroprevalence of Human Herpesvirus 8 Infection in HIV-positive Homosexual Men in Jiangsu Province, China.

作者信息

Li Jiayan, Song Jinyun, Tan Guolei, Wang Jianfang, Zhou Zhenxian, Wu Xuping

机构信息

The Second Hospital of Nanjing Affiliated to Medical School of Southeast University, Nanjing, 210003. China.

Nanjing Kindmed Clinical Laboratory,Co., Ltd., Nanjing, Jiangsu 210042. China.

出版信息

Curr HIV Res. 2017;15(4):273-278. doi: 10.2174/1570162X15666170613084343.

DOI:10.2174/1570162X15666170613084343

PMID:28606034

Abstract

BACKGROUND

HIV-infected homosexual men are more frequently diagnosed with Kaposi's sarcoma. With the increase of HIV-infected homosexual men in China, we urgently need to know the KS-related human herpesvirus (KSHV/HHV-8) seroprevalence in this population. To investigate HHV-8 prevalence among HIV-positive homosexual men, we recruited 183 patients naive of antiretroviral therapy (ART) whose blood samples presented with HIV-antibody positive as confirmed by western blot.

METHODS

HIV viral load was tested using Cobas TaqMan HIV-1 test Version 2.0, and CD4 T cell counts were tested using a Flow cytometry instrument. All HIV-positive blood samples were collected and screened for KSHV. Immunofluorescence (IFA) test was conducted for HHV-8-Specific antibodies (anti-LANA) in the plasma. HHV-8 DNA in whole blood cells of IFA-positive subjects was quantified with Real-time polymerase chain reaction (RT-PCR).

RESULTS

All samples showed HIV RNA positive. CD4+ T cell count was 23% cases (42/183) which showed ≤200 cells/μL, 51.3% cases (94/183) showed 201-500 cells/μL and 25.7% cases (47/183) showed ≥501 cells/μL. Immunofluorescence (IFA) test demonstrated an HHV-8 prevalence of 50.8% (93/183), among which 20.4% of the cases (19/93) were HHV-8 DNA positive. HHV-8 infection showed no difference among different age groups (p=0.96). Similarly, HHV-8 infection exhibited no significant difference among different HIV viral load groups (p=0.08). However, HHV-8 infection among different CD4+ T cell count showed significant difference (P=0.0004).

CONCLUSION

This study showed a high seroprevalence of human herpesvirus 8 infection in HIVpositive homosexual men.

摘要

背景

感染人类免疫缺陷病毒（HIV）的男同性恋者更常被诊断出患有卡波西肉瘤。随着中国感染HIV的男同性恋者数量增加，我们迫切需要了解该人群中与卡波西肉瘤相关的人类疱疹病毒（KSHV/HHV-8）血清流行率。为了调查HIV阳性男同性恋者中HHV-8的流行情况，我们招募了183例未接受抗逆转录病毒治疗（ART）的患者，其血液样本经蛋白质印迹法确认HIV抗体呈阳性。

方法

使用Cobas TaqMan HIV-1检测版本2.0检测HIV病毒载量，使用流式细胞仪检测CD4 T细胞计数。收集所有HIV阳性血液样本并进行KSHV筛查。对血浆中的HHV-8特异性抗体（抗LANA）进行免疫荧光（IFA）检测。对IFA阳性受试者全血细胞中的HHV-8 DNA进行实时聚合酶链反应（RT-PCR）定量。

结果

所有样本的HIV RNA均呈阳性。CD4+ T细胞计数方面，23%的病例（42/183）显示≤200个细胞/μL，51.3%的病例（94/183）显示为201 - 500个细胞/μL，25.7%的病例（47/183）显示≥501个细胞/μL。免疫荧光（IFA）检测显示HHV-8流行率为50.8%（93/183），其中20.4%的病例（19/93）HHV-8 DNA呈阳性。HHV-8感染在不同年龄组之间无差异（p = 0.96）。同样，HHV-8感染在不同HIV病毒载量组之间也无显著差异（p = 0.08）。然而，不同CD4+ T细胞计数组之间的HHV-8感染存在显著差异（P = 0.0004）。